Genetic diversity of the msp-1 locus and symptomatic malaria in south-west Nigeria
Genetic diversity of the msp-1 locus and symptomatic malaria in south-west Nigeria
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DOI：
10.1016/j.actatropica.2005.06.017
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发表时间：
2005-09-01
期刊：
影响因子：
2.7
通讯作者：
Omotade, OO
中科院分区：
文献类型：
--
作者：
Amodu, OK;Adeyemo, AA;Omotade, OO
恶性疟原虫的遗传特征可能在疟疾感染的临床严重程度中起作用。我们研究了裂殖子表面蛋白-1（msp-1）位点的多样性与尼日利亚西南部伊巴丹儿童疟疾疾病严重程度之间的关联。223名患有疟疾的儿童（中位年龄为34.5个月）入组本研究。他们包括53名儿童无症状疟疾（ASM），101名急性无并发症疟疾（UM）和69名严重疟疾（SM）。msp-1基因分型采用聚合酶链反应（PCR）方法，三组间msp-1等位基因的分布差异有统计学意义，无症状疟疾样本中位等位基因数高于其他两组。msp-1等位基因的类型与疟疾的临床类型显著相关。与无症状疟疾相比，K1等位基因的缺失与UM风险增加3倍和SM风险增加4倍相关。MAD 20等位基因的缺失与UM的生存风险增加一倍和SM增加八倍相关。我们发现尼日利亚儿童样本中恶性疟原虫的msp-1基因座与疟疾的临床严重程度之间存在关联。我们的研究结果表明，K1和MAD 20等位基因的存在与ASM显著相关，因此降低了发生症状性疾病的风险。(c)2005 Elsevier B. V.保留所有权利。
Genetic characteristics of Plasmodium falciparum may play a role in the clinical severity of malaria infection. We have studied the association between diversity at the merozoite surface protein- 1 (msp-1) locus and the severity of disease in childhood malaria in Ibadan, south-west Nigeria. Two hundred and twenty-three children (median age of 34,5 months) presenting with malaria were enrolled into the study. They comprised 53 children with asymptomatic malaria (ASM), 101 with acute uncomplicated malaria (UM) and 69 with severe malaria (SM). Genotyping of the msp-1 locus was by polymerase chain reaction.The distribution of msp-1 alleles was significantly different between the three groups, Asymptomatic malaria samples had a higher median number of alleles than the other two groups. The type of msp-1 allele detected was significantly associated with the clinical category of malaria. The absence of K1 alleles was associated with a three-fold increase risk of UM and a four-fold increased risk of SM when compared with asymptomatic malaria. The absence of MAD20 alleles was associated witha live-fold increase risk of UM and an eight-fold increase of SM.We have found an association between the msp-1 locus of P falciparum and clinical severity of malaria in a sample of Nigerian children. Our findings show that the presence of the K1 and MAD20 alleles was significantly associated with ASM and consequently a reduced risk of developing the symptomatic disease. (c) 2005 Elsevier B.V. All rights reserved.