Mass-marking of ayer eggs and larvae by tetracycline-tagging of otoliths
Mass-marking of ayer eggs and larvae by tetracycline-tagging of otoliths
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DOI：
10.2331/suisan.51.903
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发表时间：
1985
影响因子：
0.4
通讯作者：
K. Tsukamoto
中科院分区：
文献类型：
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作者：
K. Tsukamoto
本研究的目的是发展一种方法，大量标记的卵和幼虫阶段的鱼，以进行现场调查的早期生活史和种群动态的鱼。用盐酸四环素（TC）溶液浸泡香鱼卵或仔鱼，在紫外光照射下，通过耳石上的荧光标记来检测标记鱼或处理卵的鱼，以确定标记香鱼卵和仔鱼的最佳TC浓度和处理时间。测试了TC浓度和处理时间的各种组合（40-44批）。结果发现，200-300 mg/l处理24-48 h对卵是合适的，200-300 mg/ml处理3-24 h对幼虫是合适的。通过在5001塑料桶中饲养标记幼虫5个月以上，检查标记保留和TC处理对存活和生长的影响。在孵化后105天内，标记很容易被识别（100%），无需任何特殊的检测准备。即使在孵化后164天，研磨或弱酸蚀刻后的保留率为100%，而在这样的处理前为83%。处理对香鱼的存活和生长没有影响。这些结果表明，该方法可以应用于海中香鱼早期生活阶段的野外调查。
The object of this study is to develop a method of mass-marking the egg and larval stages of fish in order to carry out field surveys of the early life history and population dynamics of the fish. In this method the eggs or newly hatched larvae are immersed in tetracycline hydrochloride (TC) solution and marked-fish or fish from treated eggs are detected by the presence of a fluorescent mark on otoliths under UV light.To determine the optimum TC concentration and treatment time for marking the eggs and larvae of the ayu Plecoglossus altivelis, a diadromous fish, various combinations of TC concentration and treatment time (40-44 lots) were tested. As a result, 200-300 mg/l for 24-48 h was found to be appropriate for eggs and 200-300 mg/ml for 3-24 h for the larvae. Mark retention and the effect of TC treatment on survival and growth were examined by rearing marked larvae in 5001 plastic tanks for more than 5 months. The mark was easily descernible (100%) until 105 days after hatching without any special preparation for detection. Even 164 days after hatching the percentage of retention was 100% after grinding or etching in weak acid, compared with 83% before such treatment. The treatment had no effect on the survival and growth of the ayu. These results strongly suggest that this method can be applied to field surveys on the ayu during early life stage in the sea.