THE PURIFICATION, CHARACTERIZATION, SEROLOGICAL ACTIVITY AND HEPATOTOXIC PROPERTIES OF 2 CATIONIC GLYCOPROTEINS (ALPHA-1 AND OMEGA-1) FROM SCHISTOSOMA-MANSONI EGGS
THE PURIFICATION, CHARACTERIZATION, SEROLOGICAL ACTIVITY AND HEPATOTOXIC PROPERTIES OF 2 CATIONIC GLYCOPROTEINS (ALPHA-1 AND OMEGA-1) FROM SCHISTOSOMA-MANSONI EGGS
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DOI：
10.1017/s0031182000059503
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发表时间：
1991-10-01
期刊：
影响因子：
2.4
通讯作者：
DOENHOFF, MJ
中科院分区：
文献类型：
--
作者：
DUNNE, DW;JONES, FM;DOENHOFF, MJ
严重感染曼氏沙门氏菌的 T 细胞缺失小鼠会遭受微泡肝细胞损伤，而这在受感染的免疫完整动物中是看不到的。 曼氏沙门氏菌卵 (SEA) 的 PBS 可溶部分的阳离子部分 (CEF6) 会诱导产生抗体，这些抗体在被动转移时可防止肝细胞损伤。 CEF6 含有 2 种抗原：omega-1 和 alpha-1，也被证明是一种有用的血清诊断试剂。 本文描述了 CEF6 中 2 种抗原的纯化和表征。 omega-1 是一种单体糖蛋白，pI > 9.0，分子量为 31 kDa。 α-1 由两个免疫交叉反应二聚体组成，在非还原条件下分别为 41 和 36 kDa，每个二聚体由一种独特的和一种常见的糖蛋白亚成分组成。 在小鼠和人类感染血清 ELISA 中，omega-1 显示为曼氏血吸虫特异性，并且比普通 SEA、CEF6 或 alpha-1 能够更好地区分曼氏血吸虫感染和其他血吸虫感染。 将单特异性抗 omega-1 血清被动转移到曼氏沙门氏菌感染、T 细胞剥夺的小鼠体内，完全阻止了这些动物中微泡肝细胞损伤的发生。 单特异性抗α-1血清没有保肝能力。
T cell-deprived mice acutely infected with S. mansoni suffer microvesicular hepatocyte damage which is not seen in infected, immunological intact animals. A cationic fraction (CEF6) of the PBS-soluble portion of S. mansoni eggs (SEA) induces antibodies which, on passive transfer, prevent hepatocyte damage. CEF6 contains 2 antigens, omega-1 and alpha-1, and has also been shown to be a useful serodiagnostic reagent. This paper describes the purification and characterization of the 2 antigens present in CEF6. omega-1 is a monomeric glycoprotein with a pI > 9.0 and a molecular weight of 31 kDa. alpha-1 consists of two immunologically cross-reactive dimers, 41 and 36 kDa in non-reducing conditions, each of which consists of one unique and one common glycoprotein subcomponent. In ELISA with mouse and human infection sera omega-1 is shown to be S. mansoni specific and is better able to distinguish S. mansoni infections from other schistosome infections than are unfractionated SEA, CEF6 or alpha-1. Passive transfer of monospecific anti-omega-1 sera into S. mansoni infected, T cell-deprived mice completely prevented the occurrence of microvesicular hepatocyte damage in these animals. Monospecific anti-alpha-1 serum had no hepatoprotective capacity.