Synthetic Escherichia coli consortia engineered for syntrophy demonstrate enhanced biomass productivity
Synthetic Escherichia coli consortia engineered for syntrophy demonstrate enhanced biomass productivity
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DOI：
10.1016/j.jbiotec.2011.10.001
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发表时间：
2012-01-01
影响因子：
4.1
通讯作者：
Carlson, Ross P.
中科院分区：
文献类型：
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作者：
Bernstein, Hans C.;Paulson, Steven D.;Carlson, Ross P.
合成大肠杆菌财团工程合成营养表现出增强的生物质生产力相对于单一的文化。二元财团的目的是模仿一个无处不在的，自然发生的生态模板的初级生产力支持的二次消费。合成的聚生体通过结合葡萄糖阳性的E。大肠杆菌菌株作为系统的初级生产者，与葡萄糖阴性的E.大肠杆菌菌株，其消耗来自初级生产者的代谢副产物。工程财团利用战略分工，同时优化多个任务，提高整体文化绩效。聚生体的相互作用导致了增强的系统生物量生产力的紧急属性，这是证明了与三个不同的培养系统：批量，恒化器和生物膜生长。对于这三种培养系统，与适当的单一培养对照相比，基于葡萄糖的生物质生产率分别增加了15%、20%和50%。有趣的是，聚生体相互作用也产生了具有可预测的、自组装的、层压的微结构的生物膜。本研究建立了一个代谢工程范式，可以很容易地适应现有的E。大肠杆菌为基础的生物过程，以提高生产力的基础上强大的生态主题。(C)2011 Elsevier B.V.保留所有权利。
Synthetic Escherichia coli consortia engineered for syntrophy demonstrated enhanced biomass productivity relative to monocultures. Binary consortia were designed to mimic a ubiquitous, naturally occurring ecological template of primary productivity supported by secondary consumption. The synthetic consortia replicated this evolution-proven strategy by combining a glucose positive E. coli strain, which served as the system's primary producer, with a glucose negative E. coli strain which consumed metabolic byproducts from the primary producer. The engineered consortia utilized strategic division of labor to simultaneously optimize multiple tasks enhancing overall culture performance. Consortial interactions resulted in the emergent property of enhanced system biomass productivity which was demonstrated with three distinct culturing systems: batch, chemostat and biofilm growth. Glucose-based biomass productivity increased by similar to 15, 20 and 50% compared to appropriate monoculture controls for these three culturing systems, respectively. Interestingly, the consortial interactions also produced biofilms with predictable, self-assembling, laminated microstructures. This study establishes a metabolic engineering paradigm which can be easily adapted to existing E. coli based bioprocesses to improve productivity based on a robust ecological theme. (C) 2011 Elsevier B.V. All rights reserved.