Repair of DNA O-alkylation damage by various human organs.
Repair of DNA O-alkylation damage by various human organs.
复制标题
修复人体各器官的DNA O-烷基化损伤。
DOI：
10.1016/0006-291x(85)90946-5
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
D'Ambrosio,SM
中科院分区：
文献类型：
--
作者：
Wani,AA;Wani,G;D'Ambrosio,SM
Protein extracts from human adult liver, fetal liver, intestine, brain, kidney, lung and skin were tested against poly (dT) methylated· poly (dA), poly (dA) methylated· poly (dT) and methylated DNA. The suitability of various substrates was established in assays using E. coli extracts that removed O 4-methylthymidine (O 4-MedT), O 2-MedT, and O 6-methylguanine (O 6-MeG). The human extracts efficiently removed O 6-MeG and N3-methyladenine from methylated substrates. The adult liver exhibited low and fetal tissues negligible removal of O 4-MedT. Only the liver showed limited removal of O 2-MedT. The poor removal of the miscoding base O 4-MedT by human organs could be an important factor in carcinogen induced mutagenesis, carcinogenesis and teratogenesis.
