Emergence of a new lumpy skin disease virus variant in Kurgan Oblast, Russia, in 2018
Emergence of a new lumpy skin disease virus variant in Kurgan Oblast, Russia, in 2018
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DOI：
10.1007/s00705-020-04607-5
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发表时间：
2020-04-11
影响因子：
2.7
通讯作者：
Alexander, Sprygin
中科院分区：
文献类型：
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作者：
Aleksandr, Kononov;Pavel, Prutnikov;Alexander, Sprygin
在本文中，我们报告了2018年俄罗斯库尔干州块状皮肤病（LSD）的复苏。大多数暴发是无声的，没有死亡，集中在距离哈萨克斯坦国家边界1-50公里的半径约30公里的区域内。在初步分子诊断后，对LSD病毒（LSDV）分离株进行了针对不同基因位点的PCR分析，即LSD008（疫苗）、LSDV126（田间）和GPCR（疫苗和田间），以进行分化和基因型分配。所有分离株均为GPCR疫苗基因型阳性，其他靶病毒检测均为阴性。利用LSD008进行的熔体曲线PCR分析表明，菌株的熔体曲线与野外菌株相似。令人惊讶的是，RPO30和GPCR基因的序列分析显示，Kurgan/2018分离物在GPCR位点与KSGP O-240一致，而在RPO30位点与Saratov/2017一致。后者与由南非KSGP O-240菌株和NI-2490菌株组成的野外菌株亚群形成关联。由于这些不一致的系统发育模式，研究了另外三个位点ORF19 （kelch样蛋白）、ORF52（假定的转录延伸因子）和ORF87 （mutT motif蛋白）的序列。对这些额外基因座的系统发育分析表明，菌株Kurgan/2018属于疫苗组或现场组，强烈表明存在一种新的重组基因。这是另一个证据，揭示了卡波病毒重组的可能性，以及使用抗LSD的同源活疫苗被忽视的危险。讨论了修改以pcr为基础的区分受感染动物和接种动物策略的必要性。本文还讨论了KSGP/ ni -2490样菌株入侵的潜在情况以及对最近发现的疫苗样重组病毒的贡献。
In this paper, we report the resurgence of lumpy skin disease (LSD) in Kurgan Oblast, Russia, in 2018. The majority of the outbreaks were silent with no mortality and congregated within an area with a radius of about 30 km located 1-50 km away from the national border with Kazakhstan. Following primary molecular diagnosis, LSD virus (LSDV) isolates were analyzed using a panel of PCR assays targeting different genetic loci, namely, LSD008 (vaccine), LSDV126 (field), and GPCR (vaccine and field), for differentiation and genotype assignment. All isolates were positive for the vaccine genotype of GPCR and negative for the other field targets tested. A PCR assay with melt curve analysis utilizing LSD008, developed in this work, indicated that the strains melted with a profile similar to those of field strains. Surprisingly, sequence analysis of the RPO30 and GPCR genes aligned the Kurgan/2018 isolate with KSGP O-240 at the GPCR locus, but with Saratov/2017 at the RPO30 locus. The latter cluster forms an association with a sub-cluster of the field strains comprising the South African KSGP O-240 strain and NI-2490 strain. Due to these incongruent phylogenetic patterns, the sequences of three additional loci ORF19 (Kelch-like protein), ORF52 (putative transcriptional elongation factor), and ORF87 (mutT motif protein) were investigated. Phylogenetic analysis of these additional loci placed the strain Kurgan/2018 in either vaccine or field groups, strongly suggesting a novel recombinant profile. This is another piece of evidence exposing the potential for recombination in capripoxviruses and the ignored danger of using live homologous vaccines against LSD. The necessity to revise the PCR-based strategy differentiating infected from vaccinated animals is discussed. The potential scenarios of incursion and the contribution of the KSGP/NI-2490-like strain to the emergence of the recently identified vaccine-like recombinant are discussed.