Adhesion of streptococci to titanium and zirconia
Adhesion of streptococci to titanium and zirconia
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DOI：
10.1371/journal.pone.0234524
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发表时间：
2020-06-24
期刊：
影响因子：
3.7
通讯作者：
Yajima, Yasutomo
中科院分区：
文献类型：
--
作者：
Oda, Yukari;Miura, Tadashi;Yajima, Yasutomo
本研究的目的是评价链球菌对钛（商业纯钛：CpTi）和氧化锆（四氧化锆多晶：TZP）圆盘的粘附性。使用具有镜面抛光表面的CpTi和氧化钇稳定的TZP盘作为试样。测量了实验样品的算术平均表面粗糙度（Ra和Sa）和表面润湿性。采用X射线光电子能谱（XPS）分析实验样品的最外层，以血链球菌（Streptococcus sanguinis）、戈登链球菌（S.gordonii）、口腔链球菌（S.oralis）和变形链球菌（S. mutans）为链球菌菌株。这些细菌培养物在CpTi和TZP上生长24小时。使用ATP-生物发光测定法估计细菌粘附的数量，并对粘附的细菌标本进行扫描电子显微镜（SEM）观察。CpTi和TZP之间的表面粗糙度或润湿性没有显着差异。在XPS分析中，CpTi的最外层包括Ti 0和Ti 4+，并且TZP的最外层包括Zr 4+。在细胞粘附试验中，血链球菌、戈登链球菌和口腔链球菌对TZP的粘附力显著低于对CpTi的粘附力（p < 0.05），而变形链球菌对TZP的粘附力无显著性差异。变形链球菌对CpTi和TZP的粘附性显著低于血链球菌、戈登链球菌和口腔链球菌。血链球菌、戈登链球菌和口腔链球菌对TZP的粘附性低于对CpTi的粘附性，而变形链球菌对TZP和CpTi的粘附性相似，变形链球菌的粘附性低于其他链球菌。
The purpose of this study was to evaluate the adherence of streptococci to disks of titanium (commercially pure titanium: CpTi) and zirconia (tetragonal zirconia polycrystals: TZP). CpTi and yttria-stabilized TZP disks with a mirror-polished surface were used as specimens. The arithmetic mean surface roughness (Ra and Sa) and the surface wettability of the experimental specimens were measured. For analyzing the outermost layer of the experimental specimens, X-ray photoelectron spectroscopy (XPS) analysis was performed.Streptococcus sanguinis,S.gordonii,S.oralis, andS.mutanswere used as streptococcal bacterial strains. These bacterial cultures were grown for 24 h on CpTi and TZP. The number of bacterial adhesions was estimated using an ATP-bioluminescent assay, and scanning electron microscope (SEM) observation of the adhered bacterial specimens was performed. No significant differences in surface roughness or wettability were found between CpTi and TZP. In XPS analyses, outermost layer of CpTi included Ti0 and Ti4+, and outermost layer of TZP included Zr4+. In the cell adhesion assay, the adherences ofS.sanguinis,S.gordonii, andS.oralisto TZP were significantly lower than those to CpTi (p < 0.05); however, significant difference was not observed forS.mutansamong the specimens. The adherence to CpTi and TZP ofS.mutanswas significantly lower than that ofS.sanguinis,S.gordonii, andS.oralis. These results were confirmed by SEM.S.sanguinis,S.gordonii, andS.oralisadhered less to TZP than to CpTi, but the adherence ofS.mutanswas similar to both surfaces.S.mutanswas less adherent compare with the other streptococci tested in those specimens.