Stimuli-responsive hydrogel-silver nanoparticles composite for development of localized surface plasmon resonance-based optical biosensor
Stimuli-responsive hydrogel-silver nanoparticles composite for development of localized surface plasmon resonance-based optical biosensor
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DOI：
10.1016/j.aca.2008.01.078
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发表时间：
2008-03-24
影响因子：
6.2
通讯作者：
Hatsuzawa, Takeshi
中科院分区：
文献类型：
--
作者：
Endo, Tatsuro;Ikeda, Ryuzoh;Hatsuzawa, Takeshi
本文介绍了一种基于局部表面等离子体共振(LSPR)的、基于刺激响应水凝胶-纳米银复合材料的光学酶生物传感器。将葡萄糖氧化酶(GOx)固定在刺激响应水凝胶中，构建了该光学酶生物传感器。当将葡萄糖等样品溶液施加到该光学酶生物传感器的表面时，银纳米粒子在刺激响应水凝胶中的粒子间距增加，从而降低了LSPR的吸收强度。此外，酶反应产生的过氧化氢通过过氧化氢的分解诱导了高团簇纳米银的降解。因此，可以观察到依赖于葡萄糖浓度的LSPR吸光度的剧烈变化。在上述机理的基础上，对基于LSPR的光学酶生物传感器进行了表征。研究发现，基于LSPR的光学酶生物传感器可用于葡萄糖浓度的特异性测定。此外，该生物传感器的检测下限为10 pm。因此，这种基于LSPR的光学酶生物传感器有可能应用于低成本、高度简化和高灵敏度的医疗应用检测试剂盒。(C)2008爱思唯尔B.V.保留所有权利。
In this paper, the development of a localized surface plasmon resonance (LSPR)-based optical enzyme biosensor using stimuli-responsive hydrogel-silver nanoparticles composite is described. This optical enzyme biosensor was constructed by immobilizing glucose oxidase (GOx) into the stimuli-responsive hydrogel. When a sample solution such as glucose was applied to the surface of this optical enzyme biosensor, the interparticle distances of the silver nanoparticles present in the stimuli-responsive hydrogel were increased, and thus the absorbance strength of the LSPR was decreased. Furthermore, hydrogen peroxide, which was produced by the enzymatic reaction, induced the degradation of highly clustered silver nanoparticles by the decomposition of hydrogen peroxide. Hence, a drastic LSPR absorbance change, which depends on the glucose concentrations, could be observed. On the basis of the abovementioned mechanism, the characterization of the LSPR-based optical enzyme biosensor was carried out. It was found that the LSPR-based optical enzyme biosensor could be used to specifically determine glucose concentrations. Furthermore, the detection limit of this biosensor was 10pM. Therefore, this LSPR-based optical enzyme biosensor has the potential to be applied in cost-effective, highly simplified, and highly sensitive test kits for medical applications. (C) 2008 Elsevier B.V. All rights reserved.