Effective bone engineering with periosteum-derived cells
Effective bone engineering with periosteum-derived cells
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DOI：
10.1177/154405910708600113
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发表时间：
2007-01-01
影响因子：
7.6
通讯作者：
Ueda, M.
中科院分区：
文献类型：
--
作者：
Agata, H.;Asahina, I.;Ueda, M.
通过组织工程的骨增强已经引起了极大的兴趣。我们假设骨膜来源的细胞可以用来代替骨髓基质细胞（广泛使用）在骨工程，但这两种细胞类型之间的成骨潜力的差异尚不清楚。在这里，我们比较了这些细胞的成骨潜能，并研究了骨膜来源的细胞的最佳骨诱导条件。通过bFGF和BMP-2诱导两种细胞类型分化成成骨细胞。骨膜细胞比骨髓基质细胞增殖快，成骨标记物表明骨髓基质细胞比骨膜细胞更具有成骨性。然而，bFGF预处理使骨膜细胞对BMP-2更敏感，成骨能力更强。在用bFGF预处理后，用BMP-2处理的骨膜细胞移植物比骨髓基质细胞形成更多的新骨。这些数据的分析表明，bFGF和BMP-2的联合治疗可以使骨膜成为非常有用的骨再生来源。
Bone augmentation via tissue engineering has generated significant interest. We hypothesized that periosteum-derived cells could be used in place of bone marrow stromal cells (which are widely used) in bone engineering, but the differences in osteogenic potential between these 2 cell types are unclear. Here, we compared the osteogenic potential of these cells, and investigated the optimal osteoinductive conditions for periosteum- derived cells. Both cell types were induced, via bFGF and BMP-2, to differentiate into osteoblasts. Periosteal cells proliferated faster than marrow stromal cells, and osteogenic markers indicated that bone marrow stromal cells were more osteogenic than periosteal cells. However, pre-treatment with bFGF made periosteal cells more sensitive to BMP-2 and more osteogenic. Transplants of periosteal cells treated with BMP-2 after pre-treatment with bFGF formed more new bone than did marrow stromal cells. Analysis of these data suggests that combined treatment with bFGF and BMP-2 can make periosteum a highly useful source of bone regeneration.