Are cardiac G-proteins altered in rat models of hypertension?
Are cardiac G-proteins altered in rat models of hypertension?
复制标题
高血压大鼠模型中心脏 G 蛋白是否发生改变？
DOI：
10.1097/00004872-199304000-00005
复制
发表时间：
1993
影响因子：
4.9
通讯作者：
Insel,PA
中科院分区：
文献类型：
--
作者：
Michel,MC;Brodde,OE;Insel,PA
目的：为了确定心脏G蛋白数量或功能的改变是否参与了高血压状态下腺苷酸环化酶反应性的脱敏设计：定量测定四种不同高血压大鼠模型与相应正常血压大鼠心肌膜G蛋白亚单位的含量方法：我们比较了雄性和雌性成年自发性高血压大鼠（SHR）与年龄和性别匹配的Wistar-Kyoto（WKY）大鼠，来自WKY × SHR杂种F2代的最高压力四分位数的大鼠与来自最低压力四分位数的大鼠，以及一肾一夹肾性高血压与假手术大鼠。GS [α]的功能通过将心脏胆酸盐提取物重建到细胞膜中来定量，随后测量NaF刺激的腺苷酸环化酶活性。通过定量Western印迹实验和[125 I]-蛋白A检测确定免疫可检测的CS [α]、Gi [α]、Gq [α]和G [β]的量。结果：在任何研究的模型中，所研究的参数在高血压大鼠和正常血压大鼠之间均无显著差异。我们的结论是，心脏Gs，Gj或Gq的主要数量变化不是高血压状态的一般特征
Objective: To determine whether alterations in cardiac G-protein number or function, or both, are involved in the desensitization of adenylate cyclase responsiveness in the hypertensive stateDesign: Quantitation of G-protein subunits in myocardial membranes from four different rat models of hypertension in comparison with respective normotensive ratsMethods: We compared male and female adult spontaneously hypertensive rats (SHR) with age-and sex-matched Wistar-Kyoto (WKY) rats, rats from the highest-pressure quartile from an F2 generation of WKY x SHR hybrids with those from the lowest-pressure quartile, and one-kidney, one clip renal hypertensive with shamoperated rats. The function of GS [alpha] was quantitated by reconstitution of cardiac cholate extracts into cyc-cell membranes with subsequent measurement of NaF-stimulated adenylate cyclase activity. The amounts of immunodetectable CS [alpha], Gi [alpha], Gq [alpha], and G [beta] were determined from quantitative Western blotting experiments with [125l]-protein A detectionResults: None of the parameters investigated differed significantly between hypertensive and normotensive rats in any of the models investigatedConclusion: We conclude that major quantitative alterations in cardiac Gs, Gj or Gq are not a general feature of the hypertensive state