Two distinct oscillators in the rat suprachiasmatic nucleus in vitro.
Two distinct oscillators in the rat suprachiasmatic nucleus in vitro.
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DOI：
10.1073/pnas.92.16.7396
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发表时间：
1995-08
影响因子：
11.1
通讯作者：
Kazuyuki Shinohara;S. Honma;Y. Katsuno;H. Abe;K. Honma
中科院分区：
文献类型：
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作者：
Kazuyuki Shinohara;S. Honma;Y. Katsuno;H. Abe;K. Honma
在大鼠视交叉上核片培养中，同时和纵向测定了精氨酸加压素和血管活性肠肽释放的昼夜节律。在没有抗分裂药物处理的培养中，这两种多肽节律之间的时相关系相对恒定，但由于引入了通常用于减少神经胶质细胞数量的抗分裂药物，这种关系变得不同。连续监测这两个节律6天，观察抗有丝分裂处理后不同时期的细胞周期。此外，N-甲基-D-天冬氨酸在相同的培养条件下使两种多肽节律的相位发生了不同的变化。这些结果表明，精氨酸加压素和血管活性肠肽的释放受不同的昼夜节律振荡器的控制。
In the rat suprachiasmatic nucleus slice culture, circadian rhythms in the release of arginine vasopressin and vasoactive intestinal polypeptide were measured simultaneously and longitudinally. The phase relationship between the two peptide rhythms was relatively constant in the culture without a treatment of antimitotic drugs but became diverse by an introduction of antimitotics, which is generally used to reduce the number of glial cells. By monitoring the two rhythms continuously for 6 days, different periods were detected in culture with the antimitotic treatment. Furthermore, N-methyl-D-aspartate shifted the phase of the two peptide rhythms in the same culture differently. These results indicate that the arginine vasopressin and vasoactive intestinal polypeptide release are under control of different circadian oscillators.