Anti-inflammatory, anticholinesterase and antioxidant activity of leaf extracts of twelve plants used traditionally to alleviate pain and inflammation in South Africa
Anti-inflammatory, anticholinesterase and antioxidant activity of leaf extracts of twelve plants used traditionally to alleviate pain and inflammation in South Africa
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DOI：
10.1016/j.jep.2014.11.034
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发表时间：
2015-02-03
影响因子：
5.4
通讯作者：
Eloff, J. N.
中科院分区：
文献类型：
--
作者：
Dzoyem, J. P.;Eloff, J. N.
民族药理学相关性：氧化应激和炎症条件是阿尔茨海默病中枢神经系统相关的病理特征之一。传统上，药用植物被用来缓解炎症、疼痛和其他可能与阿尔茨海默病相关的症状。因此，本研究旨在测定南非传统上用于止痛和炎症的12种药用植物的体外抗炎、抗氧化和抗胆碱酯酶活性。材料和方法：采用内毒素激活的RAW 264.7巨噬细胞产生一氧化氮(NO)和15-脂氧合酶抑制法评价其抗炎活性。用改良的Ellman方法评价乙酰胆碱酯酶抑制作用。结果：在脂多糖刺激的RAW 264.7巨噬细胞中，非洲牛膝提取物对15-脂氧合酶活性的抑制作用最强，在100ug/mL时抑制率为85.92%，并呈剂量依赖关系。而银合欢萃取物在浓度为25mU g/m L时，对NO的抑制率可达97%。银合欢和大银杏对乙酰胆碱酯酶均有较强的抑制作用，IC50值分别为118 m u g/m L和160 m u g/m L。银合欢、爪哇李子和非洲棕榈树的酚类和类黄酮类化合物含量较高。与抗氧化活性的相关性不强，表明其他代谢产物可能也参与了抗氧化活性。结论：本研究结果验证了这些植物叶提取物在南非传统抗炎药物中的应用。这些植物的提取物可能在治疗由氧化应激和相关的退行性疾病引起的各种疾病方面有价值。(C)2014爱思唯尔爱尔兰有限公司。保留所有权利。
Ethnopharmacological relevance: Oxidative stress and inflammatory conditions are among the pathological features associated with the central nervous system in Alzheimer's disease. Traditionally, medicinal plants have been used to alleviate inflammation, pains and also other symptoms possibly associated with Alzheimer's disease. Therefore, the present study was designed to determine the in vitro anti-inflammatory, antioxidant and anticholinesterase activity of twelve South African medicinal plants traditionally used to alleviate pain and inflammation.Materials and Methods: Nitric oxide (NO) production in LPS-activated RAW 264.7 macrophages and 15-lipoxygenase (LOX) inhibitory assay were used to evaluate the anti-inflammatory activity. Acetylcholinesterase inhibition was assessed by using a modification of the Ellman's method. Antioxidant activity, total phenolic and total flavonoids contents were determined using standard in vitro methods.Results: The extract of Burkea africana had the highest anti-15-lipoxygenase activity with 85.92% inhibition at 100 mu g/mL All the extracts tested inhibited nitric oxide (NO) production in a dose dependant manner in LPS-stimulated RAW 264.7 macrophages. However, extracts from Leucaena leucocephala, Lippia javanica inhibited the production of NO by 97% at a concentration of 25 mu g/mL In addition, both Leucaena leucocephala and Englerophytum magaliesmontanum had strong activity against acetylcholinesterase with IC50 values of 118 mu g/mL and 160 mu g/mL respectively. Hight levels of phenolics and flavonoids were found in Leucaena leucocephala, Lippia javanica and Burkea africana. The correlation with antioxidant activities was not strong indicating that other metabolites may also be involved in antioxidant activity.Conclusions: The results obtained in this study validate the use of leaf extracts of these plants in South African traditional medicine against inflammation. Extracts of these plants species might be of value in the management of various diseases emerging from oxidative stress and related degenerative disorders. (C) 2014 Elsevier Ireland Ltd. All rights reserved.