Bioactivity and antibacterial activity of iodine-containing calcium titanate against implant-associated infection
Bioactivity and antibacterial activity of iodine-containing calcium titanate against implant-associated infection
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DOI：
10.1016/j.bioadv.2022.212952
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发表时间：
2022-05-27
影响因子：
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通讯作者：
Matsuda, Shuichi
中科院分区：
文献类型：
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作者：
Ikeda, Norimasa;Fujibayashi, Shunsuke;Matsuda, Shuichi
开发抗菌生物材料是骨科和牙科植入物领域的一大挑战。在这项研究中，我们评估了一种用于预防种植体相关感染的新型生物材料的骨结合能力和抗菌活性。我们之前曾报道过NaOH热处理提高了钛的骨结合能力，后来对钛进行了修饰，使其从钛酸钙表面释放出靶离子。碘是一种必需的营养物质，具有广谱的抗菌活性；因此，我们设计了一种能够释放碘离子的钛酸钙(Ca-I-Ti)。该材料是由一种简单的溶液，使用热处理以及廉价的设备和化学试剂制备的。种植在Ca-i-Ti上的MC3T3-E1细胞具有较高的生物活性和活力，Ca-i-Ti对甲氧西林敏感的金黄色葡萄球菌具有抗菌活性。体内生物力学和组织学实验表明，植入后8周，Ca-I-Ti具有良好的骨结合能力。在大鼠皮下感染模型中，种植体上对甲氧西林敏感的金黄色葡萄球菌比商业纯钛上的金黄色葡萄球菌减少了约95%，表明Ca-i-Ti在体内具有抗菌作用。此外，没有观察到局部或全身并发症，组织学上抑制了周围组织的活动性感染。因此，含碘钛酸钙是一种安全的生物材料，具有良好的生物活性和抗菌性能，在预防种植体相关感染方面具有潜在的潜力。
Developing antimicrobial biomaterials is a major challenge in the fields of orthopaedic and dental implants. In this study, we evaluated the bone-bonding ability and antibacterial activity of a novel biomaterial for preventing implant-associated infections. We have previously reported that NaOH heat treatment improved the bone-bonding ability of titanium, which was later modified to release target ions from the calcium titanate surface. Iodine, an essential nutrient, exhibits broad-spectrum antimicrobial activity; hence, we designed a calcium titanate that releases iodine ions (Ca-I-Ti). The material was prepared from a simple solution using heat treat-ments as well as inexpensive devices and chemical agents. MC3T3-E1 cells seeded on Ca-I-Ti displayed high degrees of bioactivity and viability, and Ca-I-Ti exhibited antibacterial activity against methicillin-susceptible Staphylococcus aureus. In vivo biomechanical and histological experiments showed that Ca-I-Ti had excellent bone-bonding ability at 8 weeks after implantation. In a subcutaneous infection model in rats, methicillin-susceptible Staphylococcus aureus on the implant was reduced by approximately 95% compared to that on commercially pure titanium, indicating that Ca-I-Ti has antibacterial effects in vivo. In addition, no local or systemic complications were observed, and active infection in the surrounding tissues was histologically inhibited. Thus, iodine-containing calcium titanate is a safe biomaterial with excellent bioactivity and antibac-terial properties, indicating its potential in preventing implant-associated infections.