[Effects of Carthamus tinctorius injection on bcl-2, caspase-3 expression related to neurons apoptosis after local cerebral ischemia].
[Effects of Carthamus tinctorius injection on bcl-2, caspase-3 expression related to neurons apoptosis after local cerebral ischemia].
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发表时间：
2004-10
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通讯作者：
Jia Luo;Zhi-ping Fang;Li-Ming Zhou;Song Lai
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作者：
Jia Luo;Zhi-ping Fang;Li-Ming Zhou;Song Lai
目的探讨红花对神经细胞凋亡bcl2、caspase 3表达的影响。方法将SD大鼠随机分为缺血对照组、大剂量组、中剂量组和低剂量组。采用线栓法制备大鼠大脑中动脉闭塞2小时，再灌注4小时或22小时。脑组织用2，3，5-三苯基三氮唑啉染色观察脑梗塞体积，石蜡包埋切片观察脑组织形态变化，免疫组织化学染色观察bcl-2和caspase-3的表达变化。结果各治疗组在不同时间段均能缩小脑梗塞体积(P<；0.05)，其中大剂量组缩小更明显(P<；0.05)。各处理组在不同时间段均可上调bcl-2表达，降低caspase-3表达，但以大剂量组作用更为明显(P<；0.05)。结论当归注射液可缩小脑梗塞体积，增加bcl-2，降低caspase-3表达。
OBJECTIVE To investigate the effects of Carthamus tinctorius on bcl-2, caspase-3 expression of apoptosis of neurons. METHOD SD rats were randomly divided into ischemia control group, large-dose group, middle-dose group and low-dose group. The middle cerebral artery of rats was occluded for 2h by inserting an introluminal molofilament, and reperfusion was then instituted for 4h or 22h. The brains were stained with 2, 3, 5-triphenylte trazolinm chloride for assessment of volume of infarction, and then embedded onto slides with paraffin for morphological assessment and immunohistochemistry was carried out to investigate the changes in bcl-2 and caspase-3. RESULT All treated groups at different times decreased the volume of infarction (P < 0.05), while large-dose group showed more distinct decrease than other groups (P < 0.05). All treated groups at different times increased bcl-2 and decreased caspase-3 expression as well, while large-dose group showed more distinct effect (P < 0.05). CONCLUSION C. tinctorius injection can reduce the volume of cerebral infarction, and increased bcl-2 and decreased caspase-3 expression.