DISSOCIATION OF IMMUNE-RESPONSES TO THE SS-A (RO) 52-KD AND 60-KD POLYPEPTIDES IN SYSTEMIC LUPUS-ERYTHEMATOSUS AND SJOGRENS-SYNDROME
DISSOCIATION OF IMMUNE-RESPONSES TO THE SS-A (RO) 52-KD AND 60-KD POLYPEPTIDES IN SYSTEMIC LUPUS-ERYTHEMATOSUS AND SJOGRENS-SYNDROME
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DOI：
10.1002/art.1780330307
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发表时间：
1990-03-01
影响因子：
--
通讯作者：
TAN, EM
中科院分区：
文献类型：
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作者：
BENCHETRIT, E;FOX, RI;TAN, EM
细胞核糖核酸颗粒SS-A(Ro)是许多干燥S综合征(SS)和系统性红斑狼疮(SLE)患者自身免疫反应的靶点。最近的免疫学、生化和DNA克隆研究表明，SS)A颗粒至少由两个多肽组成，分别为52kd和60kd。免疫扩散法检测60例原发性SS患者血清SS-A沉淀素阳性者47例(78%)。免疫印迹结果显示有3组反应：22例(47%)同时具有抗60kd和52kd多肽的自身抗体，19例(40%)仅与52kd蛋白反应，6例(13%)免疫扩散阳性，但在免疫印迹试验中无反应。免疫扩散法检测90例SLE患者血清SS-A沉淀素阳性者51例(57%)。在Western blotts中，24例(47%)同时具有针对60kd和52kd抗原的抗体，而9例(18%)仅与60kd蛋白反应。18例(35%)免疫印迹检测为无反应，免疫扩散检测为阳性。抗52-kd抗原抗体而不伴有60-kd抗原抗体仅见于原发SS患者，而抗60-kd抗原抗体而不伴发抗52-kd抗原抗体仅见于SLE患者。虽然SS-A抗体在SS和SLE中均可检测到，但我们的发现表明，对SS-A颗粒的两种成分抗原的免疫反应是分离的，这可能是这两种疾病启动自身免疫过程的不同事件的证据。
The cellular RNA particle SS-A (Ro) is a target of autoimmune response in many patients with Sjogren''s syndrome (SS) and systemic lupus erythematosus (SLE). Recent immunologic, biochemical, and DNA cloning studies have shwon that the SS)A particle consists of at least 2 polypeptide components, of 52 kd and 60 kd. Immunodiffusion analysis of 60 sera from patients with primary SS revealed 47 (78%) to be SS-A precipitin positive. Western blotting studies of the sera showed 3 groups of reactivities: 22 (47%) possessed autoantibodies against both the 60-kd and the 52-kd polypeptides, 19 (40%) reacted only with the 52-kd protein, and 6 (13%) were nonreactive in Western blots although positive in immunodiffusion. Fifty-one of 90 SLE sera (57%) were SS-A precipitin positive by immunodiffusion. In Western blots, 24 (47%) possessed antibodies against both the 60-kd and the 52-kd antigens, while 9 (18%) reacted only with the 60-kd protein. Eighteen (35%) were nonreactive by Western blot, although positive by immunodiffusion. Antibody to the 52-kd antigen without concomitant antibody to the 60-kd antigen was seen only in patients with primary SS, whereas antibody to the 60-kd antigen without concomitant antibody to the 52-kd antigen was seen only in SLE patients. Although antibodies to SS-A are detected in both SS and SLE, our findings show that there is dissociation of immune responses to the 2 component antigens of the particle, which may be evidence of different events initiating the autoimmune process in these diseases.