Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) contains two functional lytic origins of DNA replication
Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) contains two functional lytic origins of DNA replication
复制标题
DOI：
10.1128/jvi.76.15.7890-7896.2002
复制
发表时间：
2002-08-01
影响因子：
5.4
通讯作者：
Pari, GS
中科院分区：
文献类型：
--
作者：
AuCoin, DP;Colletti, KS;Pari, GS
我们使用瞬时转染法复制鉴定了人类疱疹病毒8型(HHV8)裂解DNA复制起点(OriLyt)的两个功能拷贝。将含有假定裂解来源的HHV8亚基因组片段与表达病毒反式激活因子开放阅读框架(ORF)50的质粒一起导入BCLB-1细胞。HHV8基因的左端oriLyt(oriLyt-L)位于ORF K4.2和K5之间，由编码多种转录因子结合位点的区域、富含A+T的区域和G+C重复区域组成。其右端oriLyt(oriLyt-R)位于ORF69和vFlIP之间，与RRV oriLyt相似，是oriLyt-L的反向重复序列。
We used a transient-transfection replication assay to identify two functional copies of the human herpesvirus 8 (HHV8) lytic origin of DNA replication (oriLyt). BCLB-1 cells were transfected with HHV8 subgenomic fragments containing the putative lytic origin along with a plasmid expressing viral transactivator open reading frame (ORF) 50. The HHV8 left-end oriLyt (oriLyt-L) lies between ORFs K4.2 and K5 and is composed of a region encoding various transcription factor binding sites and an A+T-rich region and a G+C repeat region. The right-end oriLyt (oriLyt-R) maps between ORF 69 and vFLIP, a region similar to the RRV oriLyt, and is an inverted duplication of oriLyt-L.