Molecular characterization of breast cancer CTCs associated with brain metastasis.
Molecular characterization of breast cancer CTCs associated with brain metastasis.
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DOI：
10.1038/s41467-017-00196-1
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发表时间：
2017-08-04
影响因子：
16.6
通讯作者：
Marchetti D
中科院分区：
文献类型：
--
作者：
Boral D;Vishnoi M;Liu HN;Yin W;Sprouse ML;Scamardo A;Hong DS;Tan TZ;Thiery JP;Chang JC;Marchetti D
EpCAM阳性循环肿瘤细胞（CTC）的计数允许估计乳腺癌患者中的总体转移负荷。然而，彻底了解与乳腺癌脑转移（BCBM）相关的CTC对于早期识别和评估对BCBM的治疗反应是必要的。在这里，我们报告BCBM CTC富集在可通过其生物标志物表达和突变含量识别的不同细胞亚群中。通过对CTC转录组的综合分析，我们发现了一种独特的“循环肿瘤细胞基因特征”，与原发性乳腺癌组织不同。循环肿瘤细胞基因特征的进一步剖析鉴定了与BCBM CTC相关的信号传导途径，其可能在增强BCBM中起作用。本研究提出了与BCBM有关的CTC生物标志物和信号通路，可用作脑微转移检测的筛选工具，或用于BCBM患者的合理治疗决策和监测治疗反应。来自患有脑转移的乳腺癌患者（BCBM）的CTC的表征可以允许脑转移的早期诊断和/或帮助治疗选择及其功效。在这项研究中，作者基于患者来源的CTC转录组鉴定了BCBM-CTC的独特特征，其不同于原发性肿瘤。
The enumeration of EpCAM-positive circulating tumor cells (CTCs) has allowed estimation of overall metastatic burden in breast cancer patients. However, a thorough understanding of CTCs associated with breast cancer brain metastasis (BCBM) is necessary for early identification and evaluation of treatment response to BCBM. Here we report that BCBM CTCs is enriched in a distinct sub-population of cells identifiable by their biomarker expression and mutational content. Deriving from a comprehensive analysis of CTC transcriptomes, we discovered a unique “circulating tumor cell gene signature” that is distinct from primary breast cancer tissues. Further dissection of the circulating tumor cell gene signature identified signaling pathways associated with BCBM CTCs that may have roles in potentiating BCBM. This study proposes CTC biomarkers and signaling pathways implicated in BCBM that may be used either as a screening tool for brain micro-metastasis detection or for making rational treatment decisions and monitoring therapeutic response in patients with BCBM. Characterization of CTCs derived from breast cancer patients with brain metastasis (BCBM) may allow for early diagnosis of brain metastasis and/or help for treatment choice and its efficacy. In this study, the authors identify a unique signature, based on patient-derived CTCs transcriptomes, for BCBM- CTCs that is different from primary tumors.
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影响因子：
3.7
作者：
Balasubramanian P;Lang JC;Jatana KR;Miller B;Ozer E;Old M;Schuller DE;Agrawal A;Teknos TN;Summers TA Jr;Lustberg MB;Zborowski M;Chalmers JJ
通讯作者：
Chalmers JJ
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
DOI：
10.1038/nrm3591
发表时间：
2013-06
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
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使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
--
影响因子：
64.8
作者：
Jordan, Nicole Vincent;Bardia, Aditya;Wittner, Ben S.;Benes, Cyril;Ligorio, Matteo;Zheng, Yu;Yu, Min;Sundaresan, Tilak K.;Licausi, Joseph A.;Desai, Rushil;O'Keefe, Ryan M.;Ebright, Richard Y.;Boukhali, Myriam;Sil, Srinjoy;Onozato, Maristela L.;Iafrate, Anthony J.;Kapur, Ravi;Sgroi, Dennis;Ting, David T.;Toner, Mehmet;Ramaswamy, Sridhar;Haas, Wilhelm;Maheswaran, Shyamala;Haber, Daniel A.
通讯作者：
Haber, Daniel A.