Effect of di(n-butyl) phthalate on testicular oxidative damage and antioxidant enzymes in hyperthyroid rats
Effect of di(n-butyl) phthalate on testicular oxidative damage and antioxidant enzymes in hyperthyroid rats
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DOI：
10.1002/tox.20259
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发表时间：
2007-06-01
影响因子：
4.5
通讯作者：
Kim, Hyung Sik
中科院分区：
文献类型：
--
作者：
Lee, Ena;Ahn, Mee Young;Kim, Hyung Sik
本研究比较了邻苯二甲酸二丁酯（DBP）对甲亢大鼠睾丸氧化损伤及抗氧化酶活性的影响。通过腹腔注射三碘甲状腺原氨酸（T3，10 μ g/kg体重）30天，在青春期雄性大鼠中诱导甲状腺功能亢进。正常或甲状腺功能亢进（T3）大鼠在30天内同时经口给予DBP（750 mg/kg）。与对照组相比，在甲亢组（T3，T3 + DBP）中未观察到体重变化。甲亢大鼠血清T3水平显著高于对照组，但血清促甲状腺激素水平显著低于对照组。DBP显著降低正常（DBP）和甲亢（T3 + DBP）组的睾丸重量。单纯DBP组血清睾酮浓度明显低于对照组。DBP使睾丸8-羟基脱氧鸟苷（8-OHdG）含量显著增加，而T3 + DBP组睾丸8-OHdG含量略高于DBP组。DBP组和T3 + DBP组睾丸超氧化物歧化酶和谷胱甘肽过氧化物酶活性显著升高。DBP处理组的过氧化氢酶活性显著高于T3 + DBP组，但T3 + DBP组显示出略低的DBF诱导的CAT活性。DBP组睾丸甲状腺激素受体α-1（TR α-1）表达显著升高，DBP治疗组未检测到雄激素受体（AR）表达。此外，DBP显著增加睾丸中过氧化物酶体增殖物激活受体-r（PPAR-r）水平。提示甲亢可引起睾丸PPAR-r表达水平的改变，并可能增加DBP代谢活化引起的氧化损伤水平。(c)2007 Wiley Periodicals，Inc.
This study compared the effects of di(n-butyl) phthalate (DBP) on the oxidative damage and antioxidant enzymes activity in testes of hyperthyroid rats. Hyperthyroidism was induced in pubertal male rats by intraperitoneal injection of triiodothyronine (T3, 10 mu g/kg body weight) for 30 days. An oral dose of DBP (750 mg/kg) was administered simultaneously to normal or hyperthyroid (T3) rats over a 30-day period. No changes in body weight were observed in the hyperthyroid groups (T3, T3 + DBP) compared with controls. There were significantly higher serum T3 levels observed in the hyperthyroid rats than in the control, but the serum thyroid stimulating hormone levels were markedly lower in the hyperthyroid rats. DBP significantly decreased the weight of the testes in the normal (DBP) and hyperthyroid (T3 + DBP) groups. The serum testosterone concentrations were significantly lower in only DBP group. DBP significantly increased the 8-hydroxy-2-deoxyguanosine (8-OHdG) level in the testes, whereas the DBD-incluced 8-OHdG levels were slightly higher in T3 + DBP group. Superoxide dismutase and glutathione peroxidase activities were significantly higher in the testes of the DBP or T3 + DBP groups. Catalase (W activity was significantly higher in the DBP treatment group, but the T3 + DBP group showed slightly lower DBF-induced CAT activity. The testicular expression of thyroid hormone receptor alpha-1 (TR alpha-1) was significantly higher in the DBP groups, and androgen receptor (AR) expression was not detected in the DBP treatment group. In addition, DBP significantly increased the peroxisome proliferator-activated receptor-r (PPAR-r) levels in the testis. These results suggest that hyperthyroidism can cause a change in the expression level of PPAR-r in testes, and may increase the levels of oxidative damage induced by the metabolic activation of DBP. (c) 2007 Wiley Periodicals, Inc.