Properties of freshly isolated type II alveolar epithelial cells.
Properties of freshly isolated type II alveolar epithelial cells.
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新鲜分离的 II 型肺泡上皮细胞的特性。
DOI：
10.1016/0167-4889(83)90004-6
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Shapiro,DL
中科院分区：
文献类型：
--
作者：
Finkelstein,JN;Maniscalco,WM;Shapiro,DL
本文报道了用低浓度弹性蛋白酶和胰蛋白酶混合液从成年兔肺分离的Ⅱ型肺泡上皮细胞的生化特性。发现分离后立即（4小时内）研究的细胞将放射性标记的前体[U-14 C]葡萄糖、[甲基-3H]胆碱和[3 H]棕榈酸酯掺入细胞磷脂酰胆碱的速率比先前报道的未进行短期细胞培养的细胞高2-10倍。β-肾上腺素能激动剂刺激磷脂酰胆碱的分泌。磷脂生物合成的酶在分离的肺细胞的亚细胞组分中的比活性的测量表明，一个显着富集的酰基辅酶A-溶血磷脂酰胆碱酰基转移酶，一种酶被认为是参与肺表面活性物质磷脂酰胆碱重塑，在II型细胞的内质网。这些观察结果支持的效用新鲜分离的II型细胞作为模型系统的肺泡上皮细胞的功能的研究。
The biochemical characteristics of type II alveolar epithelial cells dissociated from adult rabbit lung by instillation of low concentrations of an elastase trypsin mixture are reported. Cells studied immediately (within 4 h) after isolation were found to incorporate the radioactively labelled precursors [U-14C]glucose, [methyl-3H]choline and [3H]palmitate into cellular phosphatidylcholine at rates 2–10-fold higher than previously reported for cells not subject to short-term cell culture. Secretion of phosphatidylcholine was stimulated by beta-adrenergic agonists. Measurement of specific activities of enzymes of phospholipid biosynthesis in subcellular fractions of isolated lung cells showed a significant enrichment of acyl coenzyme A-lysophosphatidylcholine acyltransferase, an enzyme believed to be involved in pulmonary surfactant phosphatidylcholine remodeling, in the endoplasmic reticulum of type II cells. These observations support the utility of freshly isolated type II cells as a model system for the study of the functions of the alveolar epithelium.