RBC velocities in single capillaries of mouse and rat brains are the same, despite 10-fold difference in body size
RBC velocities in single capillaries of mouse and rat brains are the same, despite 10-fold difference in body size
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DOI：
10.1016/j.brainres.2010.01.032
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发表时间：
2010-03-12
期刊：
影响因子：
2.9
通讯作者：
Suzuki, Norihiro
中科院分区：
文献类型：
--
作者：
Unekawa, Miyuki;Tomita, Minoru;Suzuki, Norihiro
我们之前采用高速相机激光扫描共聚焦显微镜和红细胞跟踪软件，表明大鼠大脑皮层实质内毛细血管中的红细胞速度分布范围很广。在目前的工作中，我们测量了体重不到大鼠十分之一的小鼠的红细胞速度。在异氟烷麻醉的小鼠中，在左颞顶叶区域打开一个颅窗。静脉注射 FITC 标记的红细胞会以 500 fps 的速度自动识别和跟踪，并且所有识别的红细胞的速度均使用我们的 Matlab 域软件 KEIO-IS2 进行计算。在 21 只小鼠的 ROI 中检测到的 15241 个红细胞中，有 1655 个被确定为在毛细血管中流动。这些红细胞的速度范围为 0.15 至 8.6 mm/s，平均值为 2.03 +/- 1.42 mm/s。频率分布图显示，红细胞速度聚集在 1.0 mm/s 左右，拖尾高达 8.6 mm/s，毛细血管中 59% 的红细胞速度在 0.5 至 2.0 mm/s 范围内。出乎意料的是，尽管红细胞直径（6.0 与 6.5 μm）、体型（25 与 327 g）、心率（461 与 319 bpm）和动脉血压（86 与 84 mm Hg）存在差异，但小鼠红细胞速度的这些特征与大鼠非常相似。我们推测，与氧交换相关的物理因素可能会将毛细血管中红细胞的一般速度限制在一定范围内，以实现最佳氧交换，无论物种如何。 (C) 2010 Elsevier B.V. 保留所有权利。
Employing high-speed camera laser-scanning confocal microscopy with RBC-tracking software, we previously showed that RBC velocities in intraparenchymal capillaries of rat cerebral cortex are distributed over a wide range. In the present work, we measured RBC velocities in mice, whose body weights are less than one-tenth of that of rats. in an isoflurane-anesthetized mouse, a cranial window was opened in the left temporo-parietal region. Intravenously administered FITC-labeled RBCs were automatically recognized and tracked frame-by-frame at 500 fps, and the velocities of all RBCs recognized were calculated with our Matlab-domain software, KEIO-IS2. Among 15241 RBCs detected in the ROI in 21 mice, 1655 were identified as flowing in capillaries. The velocities of these RBCs ranged from 0.15 to 8.6 mm/s, with a mean of 2.03 +/- 1.42 mm/s. A frequency distribution plot showed that RBC velocities were clustered at around 1.0 mm/s, tailing up to 8.6 mm/s, and 59% of the RBCs in capillaries showed velocities within the range of 0.5 to 2.0 mm/s. Unexpectedly, these characteristics of RBC velocities in mice were very similar to those of rats, despite differences in RBC diameter (6.0 vs. 6.5 mu m), body size (25 vs, 327 g), heart rate (461 vs. 319 bpm) and arterial blood pressure (86 vs. 84 mm Hg). We speculate that physical factors relating to oxygen exchange may constrain general RBC velocity in capillaries to a certain range for optimum oxygen exchange, regardless of species. (C) 2010 Elsevier B.V. All rights reserved.