Effects of xylan and starch on secretome of the basidiomycete Phanerochaete chrysosporium grown on cellulose
Effects of xylan and starch on secretome of the basidiomycete Phanerochaete chrysosporium grown on cellulose
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DOI：
10.1111/j.1574-6968.2011.02307.x
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发表时间：
2011-08-01
影响因子：
2.1
通讯作者：
Samejima, Masahiro
中科院分区：
文献类型：
--
作者：
Hori, Chiaki;Igarashi, Kiyohiko;Samejima, Masahiro
木质纤维素生物质含有作为主要结构组分的纤维素和木聚糖，以及作为储存多糖的淀粉。在本研究中，我们已经使用比较分泌组学分析来研究木聚糖和淀粉对生长在纤维素上的担子菌黄孢原毛革菌分泌蛋白质表达水平的影响。采用双向电泳技术分离出47个蛋白质点，并通过液相色谱-串联质谱（LC-MS）分析鉴定。添加淀粉的纤维素分解培养物并没有影响真菌的生长显着，但减少生产的总胞外酶，包括纤维素酶和木聚糖酶。相比之下，木聚糖的加入增加了菌丝体体积和胞外蛋白的产生。木聚糖增加了几个糖苷水解酶（GH）家族10推定的内切木聚糖酶和一个推定的葡萄糖醛酸酯酶属于碳水化合物酯酶家族15，其中植物细胞壁木聚糖可能是一个底物的合成。此外，纤维二糖脱氢酶和GH家族61蛋白，这是已知的促进纤维素降解，也增加了木聚糖的存在下。这些酶可能不仅有助于真菌降解纤维素，还有助于降解植物细胞壁的复杂碳水化合物成分。
Lignocellulosic biomass contains cellulose and xylan as major structural components, and starch as a storage polysaccharide. In the present study, we have used comparative secretomic analysis to examine the effects of xylan and starch on the expression level of proteins secreted by the basidiomycete Phanerochaete chrysosporium grown on cellulose,. Forty-seven spots of extracellular proteins expressed by P. chrysosporium separated by two-dimensional electrophoresis were identified by liquid chromatography-tandem mass spectrometry analysis. Addition of starch to the cellulolytic culture did not affect fungal growth significantly, but did decrease the production of total extracellular enzymes, including cellulases and xylanases. In contrast, addition of xylan increased mycelial volume and the production of extracellular proteins. Xylan increased synthesis of several glycoside hydrolase (GH) family 10 putative endoxylanases and a putative glucuronoyl esterase belonging to carbohydrate esterase family 15, for which plant cell wall xylan may be a substrate. Moreover, cellobiose dehydrogenase and GH family 61 proteins, which are known to promote cellulose degradation, were also increased in the presence of xylan. These enzymes may contribute to degradation by the fungus of not only cellulose but also complex carbohydrate components of the plant cell wall.