MRSA detection: comparison of two molecular methods (BD GeneOhm® PCR assay and Easy-Plex) with two selective MRSA agars (MRSA-ID and Oxoid MRSA) for nasal swabs
MRSA detection: comparison of two molecular methods (BD GeneOhm® PCR assay and Easy-Plex) with two selective MRSA agars (MRSA-ID and Oxoid MRSA) for nasal swabs
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MRSA 检测：鼻拭子的两种分子方法（BD GeneOhm® PCR 检测和 Easy-Plex）与两种选择性 MRSA 琼脂（MRSA-ID 和 Oxoid MRSA）的比较
DOI：
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发表时间：
2008
影响因子：
4.5
通讯作者：
J. Harkness
中科院分区：
文献类型：
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作者：
S. V. van Hal;Z. Jennings;D. Stark;D. Marriott;J. Harkness
筛查患者是遏制耐甲氧西林金黄色葡萄球菌（MRSA）的重要组成部分。本研究的目的是确定实用的分子方法相比，选择性琼脂MRSA检测。使用显色选择性琼脂（MRSA ID和CMRSA）以及BD GeneOhm MRSA（以前称为IDI-MRSA）和Easy-PCR商业真实的时间PCR测定来筛选连续高风险患者（n = 200）的鼻腔MRSA定殖。分子方法的灵敏度与BD GeneOhm MRSA（86%）和Easy-ELISA（84%）相似。显色琼脂、MRSA ID和CMRSA的敏感性分别为62%和84%。敏感性受MRSA细菌谱和MRSA克隆类型的影响。除Easy-ELISA法（90%）外，所有方法的特异性均>98%。选择性琼脂的敏感性随着孵育48小时而增加，特异性相应降低。总之，MRSA的分子检测方法仍然敏感和快速，但费用较高。
Screening of patients is an important component for methicillin-resistant Staphylococcus aureus (MRSA) containment. The aim of this study was to determine the utility of molecular methods compared to selective agars for MRSA detection. Consecutive high risk patients (n = 200) were screened for nasal MRSA colonization using chromogenic selective agars (MRSA ID and CMRSA) as well as the BD GeneOhm MRSA (previously known as IDI-MRSA) and the Easy-Plex commercial real time PCR assays. The sensitivities of the molecular methods were similar to the BD GeneOhm MRSA (86%) and Easy-Plex (84%). The chromogenic agars, MRSA ID and CMRSA, showed sensitivities of 62% and 84%, respectively. Sensitivity was influenced by both the MRSA antibiograms and MRSA clonal type. The specificity was >98% in all methods except for the Easy-Plex assay (90%). Sensitivity of selective agars increased with 48 hours of incubation with a corresponding decrease in specificity. In conclusion, molecular detection methods for MRSA remain sensitive and rapid but are associated with greater expense.