Genome mapping by nonrandom anchoring: a discrete theoretical analysis.
Genome mapping by nonrandom anchoring: a discrete theoretical analysis.
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通过非随机锚定进行基因组作图：离散理论分析。
DOI：
10.1073/pnas.90.2.600
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发表时间：
1993
影响因子：
11.1
通讯作者：
Marr,TG
中科院分区：
文献类型：
--
作者：
Zhang,MQ;Marr,TG
作为我们努力构建裂变酵母裂糖酵母基因组物理图谱的一部分，我们已经对预期的进展进行了理论预测，通过岛屿覆盖的预期长度分数和锚定岛屿的预期属性（如岛屿的数量和大小）来衡量。我们的实验策略是随机构建一个克隆文库，筛选两端序列独特的克隆。该方案本质上与Palazzolo等人在计算机模拟中使用的克隆限制双序列标记位点选择方案相同[Palazzolo， m.j., Sawyer, s.a., Martin, c.h., Smoller, d.a.和Hartl， d.l. (1991) Proc. Natl。学会科学。[j]。我们实验室的模拟和正在进行的实验都表明，非随机锚定方法远优于随机锚定方法。本文提出了一个理论模型来解释模拟数据和实验数据。
As part of our effort to construct a physical map of the genome of the fission yeast Schizosaccharomyces pombe, we have made theoretical predictions for the progress expected, as measured by the expected length fraction of island coverage and by the expected properties of the anchored islands such as the number and the size of islands. Our experimental strategy is to construct a random clone library and screen the library for clones having unique sequence at both ends. This scheme is essentially the same as the clone-limited double sequence-tagged-site selection scheme which was used in a computer simulation by Palazzolo et al. [Palazzolo, M. J., Sawyer, S. A., Martin, C. H., Smoller, D. A. & Hartl, D. L. (1991) Proc. Natl. Acad. Sci. USA 88, 8034-8038]. Both simulation and ongoing experiments in our laboratory have shown that the nonrandom anchoring method is far superior to random anchoring. In this paper, we propose a theoretical model to explain the simulated data and the experimental data.