A MODIFIED URONIC ACID CARBAZOLE REACTION
A MODIFIED URONIC ACID CARBAZOLE REACTION
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DOI：
10.1016/0003-2697(62)90095-7
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发表时间：
1962-01-01
影响因子：
2.9
通讯作者：
MUIR, HM
中科院分区：
文献类型：
--
作者：
BITTER, T;MUIR, HM
本文对硼酸盐存在下糖醛酸的Dische咔唑反应进行了改进。该方法的优点是灵敏度提高了约两倍。OD是浓度在4和40 [mu]g/ml之间的线性函数。最大的颜色立即发展。颜色稳定至少16小时。重现性更好，减少氯离子和氧化剂的干扰。通过比较两种方法中氯化物对显色量的影响，可以区分肝素、肝素衍生物和其他结缔组织的多糖醛酸苷。
A modification of Dische''s carbazole reaction for uronic acid in the presence of borate is described. The advantages of the procedure are There is an approximately twofold increase of sensitivity. The OD is a linear function of concentration between 4 and 40 [mu]g/ml. Maximum color develops immediately. The color is stable for at least 16 hr. There is greater reproducibility and reduction of interference by chloride ion and oxidants. It has been found possible to distinguish between heparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures.