Yokukansan enhances the proliferation of B65 neuroblastoma.
Yokukansan enhances the proliferation of B65 neuroblastoma.
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DOI：
10.1016/j.jtcme.2016.01.006
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发表时间：
2017-01
影响因子：
4.5
通讯作者：
Takeda, Hiroshi
中科院分区：
文献类型：
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作者：
Nakatani, Yoshihiko;Amano, Taku;Yamamoto, Hikaru;Sakai, Norio;Tsuji, Minoru;Takeda, Hiroshi
日本传统草药Yokukansan被认为是治疗神经退行性疾病、神经症、失眠、阿尔茨海默病的行为和心理症状等几种神经系统疾病的一种新的替代疗法。此外，在动物模型中也显示出yokukan具有抗抑郁和缓解疼痛的作用。最近，几项研究表明，横山具有神经保护作用。在这项研究中，我们通过使用来自单胺能神经元的B65神经母细胞瘤细胞，研究了yokukansan是否会影响与细胞周期进展相关的细胞增殖。在yokukansan治疗下，B65神经母细胞瘤细胞的增殖率呈剂量依赖性显著增加。其中，横坎山对B65神经母细胞瘤细胞的增殖作用在横坎山治疗48 h和72 h后明显增强。此外，在横坎山的7种中草药中，柴胡和甘草均能促进B65神经母细胞瘤细胞的增殖。我们评估了横坎散对B65神经母细胞瘤细胞中p44/42丝裂原活化蛋白激酶（MAPK）磷酸化的影响，发现横坎散在治疗48小时后增加了p44/42 MAPK的磷酸化。相比之下，柴胡根和甘草根都没有改变p44/42 MAPK的磷酸化水平，尽管它们确实增加了细胞增殖。我们的研究结果表明，横山具有细胞增殖作用，这与p44/42 MAPK信号级联无关，这是由于柴胡和甘草的共同作用。
Yokukansan, a traditional Japanese herbal medicine, has been considered to be a novel alternative treatment for several neurological diseases such as neurodegenerative disorders, as well as neurosis, insomnia, and behavioral and psychological symptoms in Alzheimer's disease. Moreover, it has been shown that yokukansan has antidepressant-like and pain-relieving effects in animal models. Recently, several studies have shown that yokukansan has a neuroprotective effect. In this study, we focused on whether or no yokukansan influences cell proliferation related to cell-cycle progression by using B65 neuroblastoma cells derived from monoaminergic neurons. Under treatment with yokukansan, the proliferation rate of B65 neuroblastoma cells significantly increased in a dose-dependent manner. In particular, a proliferative effect was observed after treatment with yokukansan for 48 h and 72 h. Moreover, among seven medicinal herbs that comprise yokukansan, both Bupleuri Radix and Glycyrrhize Radix also enhanced the proliferation of B65 neuroblastoma cells. We assessed the effect of yokukansan on p44/42 mitogen-activated protein kinase (MAPK) phosphorylation in B65 neuroblastoma cells, and found that yokukansan increased p44/42 MAPK phosphorylation after treatment for 48 h. In contrast, neither Bupleuri Radix nor Glycyrrhize Radix altered the level of p44/42 MAPK phosphorylation, although they did increase cell proliferation. Our findings suggest that yokukansan has a cell-proliferative due to both Bupleuri Radix and Glycyrrhize Radix, and this is unrelated to the p44/42 MAPK signaling cascade.