AN ALTERED AROA GENE-PRODUCT CONFERS RESISTANCE TO THE HERBICIDE GLYPHOSATE
AN ALTERED AROA GENE-PRODUCT CONFERS RESISTANCE TO THE HERBICIDE GLYPHOSATE
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DOI：
10.1126/science.221.4608.370
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
STALKER, DM
中科院分区：
文献类型：
--
作者：
COMAI, L;SEN, LC;STALKER, DM
对除草剂草甘膦（N-膦酰甲基甘氨酸）通过抑制芳香族氨基酸前体5-烯醇式-3-磷酸莽草酸的合成而作用于植物和微生物的假设进行了测试。用甲磺酸乙酯处理鼠伤寒沙门氏菌，通过选择草甘膦抗性分离编码5-烯醇式-3-磷酸莽草酸合成酶的aroA基因座的突变体。其中一个突变体导致合成对草甘膦抑制有抗性的5-烯醇基-3-磷酸莽草酸合成酶。克隆了突变aroA基因和相应的野生型等位基因。当在存在或不存在野生型aroA等位基因的情况下引入大肠杆菌中时，该突变赋予对草甘膦的高抗性。
The hypothesis that the herbicide glyphosate (N-phosphonomethylglycine) acts on plants and microorganisms by inhibiting synthesis of 5-enolpyruvyl-3-phosphoshikimate, a precursor to aromatic amino acids, was tested. Salmonella typhimurium was treated with ethyl methanesulfonate and mutants mapping at the aroA locus, which encodes 5-enolpyruvyl-3-phosphoshikimate synthetase, were isolated by selection for glyphosate resistance. One of the mutants results in the synthesis of a 5-enolpyruvyl-3-phosphoshikimate synthetase that is resistant to inhibition by glyphosate. The mutant aroA gene and the corresponding wild-type allele were cloned. The mutation confers high resistance to glyphosate when introduced in Escherichia coli in the presence or absence of the wild-type aroA allele.