Differential gene expression in incompatible interaction between Lilium regale Wilson and Fusarium oxysporum f. sp lilii revealed by combined SSH and microarray analysis
Differential gene expression in incompatible interaction between Lilium regale Wilson and Fusarium oxysporum f. sp lilii revealed by combined SSH and microarray analysis
复制标题
百合和尖孢镰刀菌之间不相容相互作用中的差异基因表达。
DOI：
10.1134/s0026893314060144
复制
发表时间：
2014-11-01
影响因子：
1.2
通讯作者：
Chen, C.
中科院分区：
文献类型：
--
作者：
Rao, J.;Liu, D.;Chen, C.
镰刀菌枯萎病是由土传病原体尖孢镰刀菌（Fusarium oxysporum f.）百合病是百合的主要病害。为了分离抗赤霉病菌中差异表达的基因，尖孢反应。regale Wilson的cDNA文库。在F.利用抑制性消减杂交（SSH）技术对尖孢霉（oxysporum）感染的基因组DNA进行克隆，共获得585个特异表达序列标签（ESTs）。此外，还研究了L. regale和F.通过对585个独特EST的寡核苷酸微阵列分析，与感病的东方百合杂交种“西伯利亚”和尖孢百合之间的相容性相互作用进行比较，揭示了尖孢百合的遗传多样性。尖孢菌属表达谱分析结果表明，病程相关蛋白、抗氧化应激酶、次生代谢酶、转录因子、信号转导蛋白等基因以及大量未知基因参与了小麦的早期防御反应。对F.尖孢菌感染此外，以下定量逆转录PCR（QRT-PCR）分析证实了寡核苷酸微阵列数据的可靠性。在本研究中，分离差异表达基因的L。在对F.尖孢镰刀菌的研究有助于揭示L.对F.尖孢菌属
Fusarium wilt, caused by a soilborne pathogen Fusarium oxysporum f. sp. lilii, is the major disease of lily (Lilium L.). In order to isolate the genes differentially expressed in a resistant to F. oxysporum reaction in L. regale Wilson, a cDNA library was constructed with L. regale root during F. oxysporum infection using the suppression subtractive hybridization (SSH), and a total of 585 unique expressed sequence tags (ESTs) were obtained. Furthermore, the gene expression profiles in the incompatible interaction between L. regale and F. oxysporum were revealed by oligonucleotide microarray analysis of 585 unique ESTs in comparison to the compatible interaction between a susceptible Lilium Oriental Hybrid ‘Siberia’ and F. oxysporum. The result of expression profile analysis indicated that the genes encoding pathogenesis-related proteins (PRs), antioxidative stress enzymes, secondary metabolism enzymes, transcription factors, signal transduction proteins as well as a large number of unknown genes were involved in the early defense response of L. regale to F. oxysporum infection. Moreover, the following quantitative reverse transcription PCR (QRT-PCR) analysis confirmed reliability of the oligonucleotide microarray data. In the present study, isolation of differentially expressed genes in L. regale during response to F. oxysporum helped to uncover the molecular mechanism associated with the resistance of L. regale against F. oxysporum.