Genetic analysis of strains of Japanese Encephalitis Virus isolated from swine in central China
Genetic analysis of strains of Japanese Encephalitis Virus isolated from swine in central China
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DOI：
10.1007/s11262-010-0464-9
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发表时间：
2010-06-01
期刊：
影响因子：
1.6
通讯作者：
Zhang, Gai-Ping
中科院分区：
文献类型：
--
作者：
Fan, Jian-Ming;Luo, Jun;Zhang, Gai-Ping
本研究从华中地区部分猪场随机采集的流产胎儿或死胎仔猪脑脊液中分离到4株日本脑炎病毒（JEV）。用RT-PCR方法克隆E基因并测序。对国内外已报道的48株乙脑病毒分离株进行系统发育分析，结果表明，4株乙脑病毒均属于基因型III亚群。结果表明，基因型III是目前我国中部地区猪群流行的主要变异株。
In this study, four strains of Japanese Encephalitis Virus (JEV) were isolated from the cerebrospinal fluid of aborted fetuses or stillborn piglets collected randomly from a number of piggeries in central China. The E genes were cloned by RT-PCR and sequenced. Phylogenetic analysis was performed with 48 JEV isolates previously reported in China and other countries, and showed that all four isolates can be classified into the subcluster of genotype III. The results strongly suggest that the genotype III of JEV is the major variant currently circulating in the swinery of central China.