Comparative Pharmacokinetics of Seven Major Compounds in Normal and Atherosclerosis Mice after Oral Administration of Simiao Yong'an Decoction.
Comparative Pharmacokinetics of Seven Major Compounds in Normal and Atherosclerosis Mice after Oral Administration of Simiao Yong'an Decoction.
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口服四妙永安汤后七种主要化合物在正常小鼠和动脉粥样硬化小鼠体内的药动学比较
DOI：
10.1155/2022/4604601
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发表时间：
2022
影响因子：
--
通讯作者：
Nie, Bo
中科院分区：
文献类型：
--
作者：
Sun, Ke-han;Yang, Man-fang;Xu, Xin-rui;Li, Yang;Gao, Zhao;Zhang, Qing-yue;Li, Hui;Wang, Shu-qi;Lou, Li-xia;Wu, Ai-ming;Jin, Qiu-shuo;Wu, Sheng-xian;Nie, Bo
四妙勇安汤是我国治疗动脉粥样硬化（AS）的经典方剂，但其治疗机理和药效物质基础尚不清楚。本研究通过高脂饮食和颈动脉颈动脉环放置（PCCP）造成AS模型，并对模型小鼠和正常小鼠口服SMYAD。建立了一种快速、灵敏、选择性好、结果可靠的超高效液相色谱（UHPLC）-Q Exactive HF-X质谱联用（UHPLC-Q Exactive HF-X MS）方法，用于同时测定正常小鼠和AS小鼠血浆中的哈巴苷、绿原酸、当药苦苷、当药苷、安果苷C、甘草苷和异甘草素等7种化合物。该方法的专属性、线性、精密度、准确度、回收率和稳定性均在可接受标准范围内。结果表明，两组小鼠中哈巴苷、绿原酸和异甘草素的某些药代动力学行为存在显著差异。具体参数变化为哈帕格特（AUC 0-t和AUC 0-∞分别为11075.09 ± 2132.38和16221.95 ± 5622.42 ng·mL−1·h; CLz/F为2.45 ± 0.87 L/h/mg），绿原酸（t1/2为21.59 ± 9.16 h; AUC 0-∞为2637.51 ± 322.54 ng·mL−1·h; CLz/F为13.49 ± 1.81 L/h/mg）和异甘草素（AUC 0-t和AUC 0-∞分别为502.25 ± 165.65和653.68 ± 251.34 ng·mL-1·h; CLz/F为62.16 ± 23.35 L/h/mg）在AS病理状态下发生改变。这些差异可能部分归因于AS状态下胃肠道菌群、非特异性药物转运蛋白和细胞色素P450活性的变化，为AS的药理作用和药效物质基础提供了研究思路和实验依据。
Simiao Yong'an decoction (SMYAD), a classic traditional Chinese medicine formula, has been used to treat atherosclerosis (AS) in clinical in China, but its therapeutic mechanism and pharmacodynamic material basis are not clear. In this study, the AS model was caused by a high-fat diet and perivascular carotid collar placement (PCCP), and SMYAD was orally administered to the model and normal mice. A rapid, sensitive, selective, and reliable method using ultrahigh-performance liquid chromatography (UHPLC) system combined with a Q Exactive HF-X mass spectrometer (UHPLC-Q Exactive HF-X MS) was established and validated for the simultaneous determination of seven compounds, including harpagide, chlorogenic acid, swertiamarin, sweroside, angoroside C, liquiritin, and isoliquiritigenin in the plasma of normal and AS mice. The specificity, linearity, precision, accuracy, recovery, and stability of the method were all within the acceptable criteria. The results showed that some pharmacokinetic behaviors of harpagide, chlorogenic acid, and isoliquiritigenin were significantly different among the two groups of mice. The specific parameter changes were harpagide (AUC0–t and AUC0–∞ were 11075.09 ± 2132.38 and 16221.95 ± 5622.42 ng·mL−1·h, respectively; CLz/F was 2.45 ± 0.87 L/h/mg), chlorogenic acid (t1/2 was 21.59 ± 9.16 h; AUC0–∞ was 2637.51 ± 322.54 ng·mL−1·h; CLz/F was 13.49 ± 1.81 L/h/mg) and isoliquiritigenin (AUC0–t and AUC0–∞ were 502.25 ± 165.65 and 653.68 ± 251.34 ng·mL−1·h, respectively; CLz/F was 62.16 ± 23.35 L/h/mg) were altered under the pathological status of AS. These differences might be partly ascribed to the changes in gastrointestinal microbiota, nonspecific drug transporters, and cytochrome P450 activity under the AS state, providing research ideas and experimental basis for pharmacological effects and pharmacodynamic material basis.
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采用 UPLC-MS/MS 进行安果苷 C 及其代谢物阿魏酸在大鼠体内的药代动力学、生物利用度和组织分布研究
影响因子：
5.6
作者：
Zhang C;Ma W;Zhang Y;Wang Q;Qin C;Du S;Huang L;Ye F;Chen L;Zheng T
通讯作者：
Zheng T
影响因子：
7.5
作者：
Qi, Zhongwen;Li, Meng;Zhang, Junping
通讯作者：
Zhang, Junping
影响因子：
7.4
作者：
Tuteja, Sony;Ferguson, Jane F.
通讯作者：
Ferguson, Jane F.
DOI：
10.3390/molecules22111937
发表时间：
2017-11-09
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Liu Y;Chi S;Wang W;Su L;Liu B
通讯作者：
Liu B
异甘草素可减弱载脂蛋白 E 缺陷小鼠的动脉粥样硬化形成
影响因子：
5.6
作者：
Du F;Gesang Q;Cao J;Qian M;Ma L;Wu D;Yu H
通讯作者：
Yu H