In vitro studies of DNA mismatch repair proteins.
In vitro studies of DNA mismatch repair proteins.
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DOI：
10.1016/j.ab.2011.02.017
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发表时间：
2011-06-15
影响因子：
2.9
通讯作者：
Hsieh P
中科院分区：
文献类型：
--
作者：
Geng H;Du C;Chen S;Salerno V;Manfredi C;Hsieh P
The ability to monitor and characterize DNA mismatch repair activity in various mammalian cells is important for understanding mechanisms involved in mutagenesis and tumorigenesis. Since mismatch repair proteins recognize mismatches containing both normal and chemically altered or damaged bases, in vitro assays must accommodate a variety of mismatches in different sequence contexts. Here we describe the construction of DNA mismatch substrates containing G:T or O6meG:T mismatches, the purification of recombinant native human MutSα (MSH2-MSH6) and MutLα (MLH1-PMS2) proteins, and in vitro mismatch repair and excision assays that can be adapted to study mismatch repair in nuclear extracts from mismatch repair proficient and deficient cells.
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影响因子：
56.9
作者：
DRUMMOND, JT;LI, GM;MODRICH, P
通讯作者：
MODRICH, P
影响因子：
16
作者：
Yoshioka, Ken-Ichi;Yoshioka, Yoshiko;Hsieh, Peggy
通讯作者：
Hsieh, Peggy
DOI：
10.1073/pnas.76.2.655
发表时间：
1979-01-01
影响因子：
11.1
作者：
CHALLBERG, MD;KELLY, TJ
通讯作者：
KELLY, TJ
影响因子：
16
作者：
Genschel, J;Modrich, P
通讯作者：
Modrich, P
DOI：
10.1073/pnas.87.15.5837
发表时间：
1990-08-01
影响因子：
11.1
作者：
HOLMES, J;CLARK, S;MODRICH, P
通讯作者：
MODRICH, P