SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF CHLORHEXIDINE IN HUMAN-SERUM AND URINE
SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF CHLORHEXIDINE IN HUMAN-SERUM AND URINE
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DOI：
10.1016/s0378-4347(00)83482-9
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发表时间：
1986-12-19
期刊：
影响因子：
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通讯作者：
PITTMAN, KA
中科院分区：
文献类型：
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作者：
BROUGHAM, LR;CHENG, H;PITTMAN, KA
建立了分析人血清和尿液中洗必太的高效液相色谱方法。将洗必太和内标扑尔敏萃取到含有 5% 2-丙醇的氯仿中，然后反萃取到稀硫酸中。在用甲醇-水（70:30，v/v）平衡的 C18 柱上实现色谱分离，其中含有 0.005 M 庚烷磺酸钠。使用 0.5 ml 样品，测定的灵敏度为 20 ng/ml 生物基质。证明了该方法可用于监测局部用含氯己定烧伤膏治疗的烧伤患者的氯己定水平。
A high-performance liquid chromatographic method for the analysis of chlorhexidine in human serum and urine was developed. Chlorhexidine and the internal standard, chlorpheniramine, were extracted into chloroform, containing 5% 2-propanol, and back-extracted into dilute sulfuric acid. Chromatographic separation was achieved on a C18 column equilibrated with methanol-water (70:30, v/v), containing 0.005 M sodium heptanesulfonate. The sensitivity of the assay was 20 ng/ml of biological matrix, using 0.5-ml samples. The application of this method to monitor chlorhexidine levels in burn patients treated topically with a chlorhexidine-containing burn cream was demonstrated.