KISS1 expression in human female adipose tissue
KISS1 expression in human female adipose tissue
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DOI：
10.1007/s00404-012-2514-0
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发表时间：
2013-01-01
影响因子：
2.6
通讯作者：
Wilkinson, Michael
中科院分区：
文献类型：
--
作者：
Cockwell, Heather;Wilkinson, Diane A.;Wilkinson, Michael
目前Kisspeptin研究的重点是探索其在人类和动物生育的下丘脑控制中的关键作用。尽管这些研究的重要性，强有力的证据表明，基因编码人kisspeptin，KISS 1，存在于几个外周网站，包括胎盘。我们还提供了kiss 1在啮齿动物脂肪组织中也表达和调节的证据。这项研究描述了一项初步调查，人类女性脂肪组织可能也表达KISS 1基因。脂肪样本取自接受开腹手术的女性，例如，在剖腹产。获得两个小的脂肪样品，一个来自皮下组织（n = 35），一个来自网膜（n = 32）。从所有脂肪样品中分离RNA，并通过实时RT-PCR检测KISS 1 mRNA。在所有脂肪组织样品中检测到不同水平的KISS 1基因表达，但在皮下脂肪中的水平显著较高。皮下脂肪中KISS 1基因表达与体重指数（BMI）无显著相关性（P = 0.43），而大网膜脂肪中KISS 1 mRNA表达与BMI显著正相关（P = 0.01）。需要进一步的研究来确定脂肪组织来源的kisspeptins是否可能与不孕症的某些方面相关。
The current focus of kisspeptin research is an exploration of its key role in the hypothalamic control of human and animal fertility. Notwithstanding the importance of these studies, strong evidence exists that the gene encoding human kisspeptin, KISS1, is present in several peripheral sites, including the placenta. We also provided evidence that kiss1 is also expressed and regulated in rodent adipose tissue. This study describes a pilot investigation into the possibility that human female adipose tissue might also express the KISS1 gene.Samples of fat were taken from women undergoing open abdominal surgery, for example, during caesarian section. Two small samples of fat were obtained, one from subcutaneous tissue (n = 35) and one from the omentum (n = 32). RNA was isolated from all fat samples and KISS1 mRNA was detected by realtime RT-PCR.KISS1 gene expression was detected at varying levels in all samples of fat tissue but levels were significantly higher in subcutaneous fat. There was no significant correlation between KISS1 gene expression and body mass index (BMI) in subcutaneous fat (P = 0.43), but there was a significant positive correlation (P = 0.01) between KISS1 mRNA levels and BMI in omental adipose tissue.We have shown for the first time that human female adipose tissue may be a source of kisspeptins. Further studies are required to establish whether kisspeptins of adipose tissue origin might be correlated with some aspects of infertility.