Insulin-Coated Gold Nanoparticles: A Plasmonic Device for Studying Metal-Protein Interactions
Insulin-Coated Gold Nanoparticles: A Plasmonic Device for Studying Metal-Protein Interactions
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DOI：
10.1002/smll.201100735
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发表时间：
2011-09-19
期刊：
影响因子：
13.3
通讯作者：
Liz-Marzan, Luis M.
中科院分区：
文献类型：
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作者：
Chanana, Munish;Correa-Duarte, Miguel A.;Liz-Marzan, Luis M.
胰岛素包覆的金纳米粒子(Au@Insulin NPs)由于蛋白质的包裹，对pH和重金属高度敏感。在各种重金属离子存在下，Au@Insulin纳米粒子的聚集和解聚随着pH的变化而可逆地进行，这可以通过其光学性质的可逆变化来监测。纳米粒子对不同金属离子的敏感性是不同的，依赖于每个特定金属离子的配位性质、价数、浓度和反应时间(动力学)，这是研究基本金属-蛋白质相互作用的一种简单工具。此外，由于其坚固的蛋白质涂层，Au@Insulin纳米粒子在高离子强度下具有生物相容性和高度稳定性。
Insulin-capped gold nanoparticles (Au@insulin NPs) are highly sensitive towards pH and heavy metals, due to the protein coating. Au@insulin NPs aggregate and disaggregate reversibly with pH and in the presence of various heavy metal ions, which can be monitored through reversible changes in their optical properties. The sensitivity of the NPs towards different metal ions is dissimilar and depends on the coordinative properties of each specific metal ion, its valence number, concentration, and reaction time (kinetics), representing a simple tool for studying fundamental metal-protein interactions. Moreover, Au@insulin NPs are biocompatible and highly stable at high ionic strengths, due to their robust protein coating.