HOMOLOGS OF THE SYNAPTOBREVIN VAMP FAMILY OF SYNAPTIC VESICLE PROTEINS FUNCTION ON THE LATE SECRETORY PATHWAY IN SACCHAROMYCES-CEREVISIAE
HOMOLOGS OF THE SYNAPTOBREVIN VAMP FAMILY OF SYNAPTIC VESICLE PROTEINS FUNCTION ON THE LATE SECRETORY PATHWAY IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0092-8674(93)90465-3
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发表时间：
1993-09-10
期刊：
影响因子：
64.5
通讯作者：
GERST, JE
中科院分区：
文献类型：
--
作者：
PROTOPOPOV, V;GOVINDAN, B;GERST, JE
酿酒酵母拥有两个基因，SNC1和SNC2，它们编码脊椎动物突触囊泡相关膜蛋白的同源物，也被称为突触brevins。在这里，我们描述了SNC2的克隆，并证明缺乏SNC基因的酵母缺乏正常的大量分泌，积累大量的后高尔基囊泡，并表现出多种条件致死表型。此外，我们发现酵母Snc蛋白定位于高尔基转运后的囊泡，这些囊泡在晚作用的sec突变体中积累。我们的发现明确地将Snc蛋白置于酿酒酵母的分泌后期通路上，并提供了突触brevin样蛋白介导分泌蛋白靶向和转运的直接体内证据。
The yeast S. cerevisiae possesses two genes, SNC1 and SNC2, that encode homologs of vertebrate synaptic vesicle-associated membrane proteins, also known as synaptobrevins. Here we describe the cloning of SNC2 and demonstrate that yeast lacking both SNC genes are deficient in normal bulk secretion, accumulate large numbers of post-Golgi vesicles, and display a variety of conditional lethal phenotypes. In addition, we show that yeast Snc proteins localize to post-Golgi transport vesicles that accumulate in a late-acting sec mutant. Our findings clearly place the Snc proteins on the late secretory pathway in S. cerevisiae and provide direct in vivo evidence that synaptobrevin-like proteins mediate the targeting and transport of secretory proteins.