An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome.
An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome.
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DOI：
10.1007/978-1-4939-7514-3_1
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Xiao C
中科院分区：
文献类型：
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作者：
Jin HY;Xiao C
全球翻译组分析技术的最新进展使我们能够了解基因表达的翻译调控如何调节细胞功能。在本章中，我们提出了一种综合的方法，通过纯化B细胞的多体分析和核糖体分析来测量翻译体的各个方面。我们标准化了我们的方案，以便直接比较这两种方法的结果。用这两种方法对翻译组进行平行评估，可以全面了解翻译调节如何决定蛋白质输出。
Recent advances in global translatome analysis technologies enable us to understand how translational regulation of gene expression modulates cellular functions. In this chapter, we present an integrated method to measure various aspects of translatome by polysome profiling and ribosome profiling using purified B cells. We standardized our protocols to directly compare the results from these two approaches. Parallel assessment of translatome with these two approaches can generate a comprehensive picture on how translational regulation determines protein output.