The stimulatory effect of TLRs ligands on maturation of chicken bone marrow-derived dendritic cells
The stimulatory effect of TLRs ligands on maturation of chicken bone marrow-derived dendritic cells
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TLRs配体对鸡骨髓源性树突状细胞成熟的促进作用
DOI：
10.1016/j.vetimm.2013.06.014
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发表时间：
2013-09-15
影响因子：
1.8
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Liang, Jinfeng;Fu, Jia;Yang, Qian
树突状细胞（dc）在先天和适应性免疫反应的启动中都是至关重要的。TLR配体与DC表面表达的toll样受体（TLRs）结合，诱导DC成熟。研究了3种TLR配体（枯草芽孢杆菌孢子、多肌苷-多胞酸和CpG寡脱氧核苷酸）对鸡骨髓源DCs （chBM-DCs）成熟的影响。在重组鸡粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素(IL)-4的作用下培养的chbm - dc在培养7天后呈现出典型的dc形态。这些未成熟的chbm - dc上调了MHC-II和推测的CD11c的表达，但CD40和CD86共刺激分子的表达水平尚低至中等。经TLR配体刺激后，chbm - dc比未成熟chbm - dc表现出更成熟的形态表型，CD40和CD86细胞表面表达水平显著提高，并在同种异体混合淋巴细胞反应中获得了刺激幼稚T细胞增殖的能力。总之，我们的数据表明，所有三种TLR配体都是驱动chbm - dc体外成熟的强刺激，枯草芽孢杆菌孢子是最有效的。(C) 2013 Elsevier B.V.版权所有
Dendritic cells (DCs) are crucial for initiation of both innate and adaptive immune responses. TLR ligands combine with Toll-like receptors (TLRs) expressed on the DC surface and induce DC maturation. The potential effect of three types of TLR ligands (Bacillus subtilis (B. subtilis) spores, polyinosinic-polycytidylic acid and CpG oligodeoxynucleotides) on chicken bone marrow-derived DCs (chBM-DCs) maturation was studied. The chBM-DCs cultured in presence of recombinant chicken granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 displayed the typical morphology of DCs after 7 days of culture. These immature chBM-DCs up-regulated the expression of MHC-II and of the putative CD11c, but had yet low to moderate levels of the CD40 and CD86 co-stimulatory molecules. After stimulation by the TLR ligands, the chBM-DCs displayed a more mature morphologic phenotype, significantly increased the CD40 and CD86 cell surface expression levels and gained the ability to stimulate proliferation of naive T cells in the allogeneic mixed lymphocyte reaction, compared to the immature chBM-DCs. In conclusion, our data demonstrated that all three TLR ligands were strong stimuli for driving chBM-DCs maturation in vitro, with B. subtilis spores being the most efficient. (C) 2013 Elsevier B.V. All rights reserved.