Amplification and expression of the human cyclin D gene in esophageal cancer.
Amplification and expression of the human cyclin D gene in esophageal cancer.
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发表时间：
1992-05
期刊：
影响因子：
11.2
通讯作者：
Wei Jiang;S. Kahn;N. Tomita;Yu-Jing Zhang;Shih-hsin Lu;I. Weinstein
中科院分区：
文献类型：
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作者：
Wei Jiang;S. Kahn;N. Tomita;Yu-Jing Zhang;Shih-hsin Lu;I. Weinstein
11q13染色体上的hst-1和int-2基因扩增已经在超过20%的人类原发性食管癌中被发现。然而，这两个基因的转录量似乎并不可观。最近，人类周期蛋白D基因（也称为prad1）被定位到11q13位点。在这里，我们报道了20例（25%）人食管鳞状肿瘤中5例细胞周期蛋白D和hst-1基因的共扩增。我们还在两种食管癌细胞系中检测到显著水平的细胞周期蛋白D转录，尽管它们没有表达可检测量的hst-1转录。这些发现为人类食管癌细胞周期蛋白基因的扩增提供了第一个证据，并提示细胞周期蛋白D基因剂量的增加可能是食管癌发病的一个重要因素。此外，由于发现11q13位点在许多类型的人类肿瘤中被扩增，细胞周期蛋白基因扩增也可能在其他形式的人类癌症的发展中发挥重要作用。
Amplification of the hst-1 and int-2 genes on chromosome 11q13 has previously been found in over 20% of human primary esophageal cancers. However, these two genes do not appear to be transcribed in appreciable amounts. Recently, the human cyclin D gene (also referred to as prad1) has been mapped to the 11q13 locus. Here, we report coamplification of the cyclin D and hst-1 genes in 5 of 20 (25%) human squamous esophageal tumors. We also detected significant levels of cyclin D transcription in two esophageal carcinoma cell lines, even though they did not express detectable amounts of hst-1 transcription. These findings provide the first evidence for the amplification of a cyclin gene in human esophageal cancer and suggest that an increase in cyclin D gene dosage could be an important factor in the pathogenesis of esophageal cancer. Additionally, because the 11q13 locus is found to be amplified in many types of human tumors, cyclin gene amplification could also play an important role in the development of other forms of human cancer.