Dibenzocyclooctadiene Lignans from Schisandra chinensis protect primary cultures of rat cortical cells from glutamate-induced toxicity
Dibenzocyclooctadiene Lignans from Schisandra chinensis protect primary cultures of rat cortical cells from glutamate-induced toxicity
复制标题
DOI：
10.1002/jnr.20089
复制
发表时间：
2004-05-01
影响因子：
4.2
通讯作者：
Kim, YC
中科院分区：
文献类型：
--
作者：
Kim, SR;Lee, MK;Kim, YC
五味子（Schisandra chinensis Baill.; Schisandraceae）的甲醇提取物能显著降低l -谷氨酸对大鼠皮层细胞的神经毒性。从甲醇提取物中分离得到5个二苯并环二烯木脂素（脱氧五味子素、五味子素N、五味子素A、五味子素C）；然后评估其对谷氨酸诱导的神经毒性的保护作用。在五种木脂素中，脱氧五味子素、葛米素N和五味子素C显著减弱谷氨酸诱导的神经毒性：1)抑制细胞内[Ca2+]的增加；2)改善谷胱甘肽防御系统、谷胱甘肽水平和谷胱甘肽过氧化物酶活性；3)抑制细胞过氧化物的形成。提示五味子二苯并环二烯木脂素对兴奋毒素引起的氧化性神经元损伤可能具有治疗作用。(C) 2004 Wiley-Liss, Inc。
A methanolic extract of dried Schisandra fruit (Schisandra chinensis Baill.; Schisandraceae) significantly attenuated the neurotoxicity induced by L-glutamate in primary cultures of rat cortical cells. Five dibenzocyclooctadiene lignans (deoxyschisandrin, gomisin N, gomisin A, schisandrin, and wuweizisu C) were isolated from the methanolic extract; their protective effects against glutamate-induced neurotoxicity were then evaluated. Among the five lignans, deoxyschisandrin, gomisin N, and wuweizisu C significantly attenuated glutamate-induced neurotoxicity as measured by 1) an inhibition in the increase of intracellular [Ca2+]; 2) an improvement in the glutathione defense system, the level of glutathione, and the activity of glutathione peroxidase; and 3) an inhibition in the formation of cellular peroxide. These results suggest that dibenzocyclooctadiene lignans from Schisandra chinensis may possess therapeutic potential against oxidative neuronal damage induced by excitotoxin. (C) 2004 Wiley-Liss, Inc.