Enhanced expression of TGFβ protein in lymphoid organs and lung, but not in serum, of pigs infected with a European field isolate of porcine reproductive and respiratory syndrome virus
Enhanced expression of TGFβ protein in lymphoid organs and lung, but not in serum, of pigs infected with a European field isolate of porcine reproductive and respiratory syndrome virus
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DOI：
10.1016/j.vetmic.2012.02.003
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发表时间：
2012-07-06
影响因子：
3.3
通讯作者：
Carrasco, Librado
中科院分区：
文献类型：
--
作者：
Gomez-Laguna, Jaime;Rodriguez-Gomez, Irene M.;Carrasco, Librado
转化生长因子β (TGF β)是一种免疫调节细胞因子，能够调节宿主免疫反应，引起对病原体的低效率反应。从这个意义上说，该细胞因子在猪繁殖与呼吸综合征（PRRS）中的作用研究很少，报道的结果也相互矛盾。因此，本研究分析TGF β在组织（淋巴器官和肺）和血清水平的表达，研究其与PRRSV （PRRSV）表达的相关性。为了开展这项研究，用欧洲PRRSV 2982野株接种32头猪，从0 dpi到研究结束（24 dpi）依次杀死。采集血液和组织样本，检测PRRSV和TGF β的表达。从接种动物3 dpi到研究结束均检测到PRRSV，但未在接种动物血清中检测到TGF β。与对照组相比，prrsv接种猪淋巴器官和肺组织中TGF β抗原均升高。由于TGF β作为免疫反应的免疫调节细胞因子，同时也参与调节性T细胞（regulatory T cells, treg）的分化，因此在PRRS期间，TGF β在组织水平的上调可能参与了宿主免疫反应的损害，在PRRSV中观察到TGF β在肺水平的表达与PRRSV有显著的相关性。(C) 2012 Elsevier BM。版权所有。
Transforming growth factor beta (TGF beta) is an immunomodulatory cytokine which is able to modulate the host immune response eliciting an inefficient response against pathogens. In this sense, the role of this cytokine in porcine reproductive and respiratory syndrome (PRRS) has been poorly studied and the reported results are contradictory. Thus, in the present study, the expression of TGF beta was analysed both at tissue (lymphoid organs and lung) and serum level to study its correlation with the expression of PRRS virus (PRRSV). To carry out this study, 32 pigs were inoculated with the European PRRSV field isolate 2982 and sequentially killed from 0 dpi to the end of the study (24 dpi). Blood and tissue samples were collected to determine the expression of PRRSV and TGF beta. PRRSV was detected in inoculated animals from 3 dpi until the end of the study, however TGF beta was not detected in sera from inoculated animals. Contrary, an increase of TGF beta antigen was observed both in the lymphoid organs and in the lung of PRRSV-inoculated pigs when compared with control group. Since TGF beta play a role as an immunomodulatory cytokine of the immune response and also in the differentiation of regulatory T cells (Tregs), the upregulation of the TGF beta at tissue level may play a role in the impairment of the host immune response observed during PRRS, being observed a significant correlation between PRRSV and TGF beta expression at lung level. (C) 2012 Elsevier BM. All rights reserved.