Expression analysis of nuclear W2-containing homologs of eukaryotic initiation factors in rice
Expression analysis of nuclear W2-containing homologs of eukaryotic initiation factors in rice
复制标题
DOI：
10.2478/s11756-011-0101-7
复制
发表时间：
2011-08
期刊：
影响因子：
1.5
通讯作者：
P. Nie;Xiaoyu Li;Yaoguang Liu;Qunyu Zhang
中科院分区：
文献类型：
--
作者：
P. Nie;Xiaoyu Li;Yaoguang Liu;Qunyu Zhang
W2结构域是一个保守的结构域，最早发现于几个翻译起始因子的C-末端，但其作用尚不清楚。本研究在水稻（Oryza sativa L.）它们位于细胞核。分析了这三个基因在植物激素和非生物胁迫下的表达谱。OsW 2 -1的表达主要受脱落酸和细胞分裂素两类植物激素以及黑暗、干旱和高盐胁迫的影响，而OsW 2 -2和OsW 2 -3的表达均受到2，4-二氯苯氧乙酸的抑制。此外，低温胁迫下OsW 2 -2和3-吲哚丁酸、激动素和盐处理下OsW 2 -3的转录水平也略有下降。我们的研究结果首次表明，这三个W2基因在植物激素信号通路中发挥作用，OsW 2 -1也与水稻的非生物胁迫抗性相关。这表明真核细胞中的翻译起始因子同源物在细胞核和细胞质中具有不同的功能。
The W2 domain is a conserved domain found firstly at the C-terminus of several translation initiation factors, but its roles are unknown. Here we identified three W2 genes in rice (Oryza sativa L.) which were located to the nucleus. The expression profiles of the three genes in response to phytohormones and abiotic stresses were also analyzed. OsW2-1 expression was mainly affected by two classes of phytohormones, including abscisic acid and cytokinin, and some abiotic stresses such as dark, drought and hypersaline, while both OsW2-2 and OsW2-3 expression levels were down-regulated by 2,4-dichloro-phenoxyacetic acid treatment. Besides, OsW2-2 under chilling stress, and OsW2-3 under 3-indolebutyric acid, kinetin and salt treatments, also showed slight transcription reduction. Our results first suggest that the three W2 genes function in phytohormone signaling pathways, and OsW2-1 also interrelated with the resistance to abiotic stresses in rice. It implies that the homologs of translation initiation factors in nuclei have different functions from those in the cytoplasm in eukaryotic cells.