Studies on woloszynskioid dinoflagellates VI: description of Tovellia aveirensis sp. nov. (Dinophyceae), a new species of Tovelliaceae with spiny cysts
Studies on woloszynskioid dinoflagellates VI: description of Tovellia aveirensis sp. nov. (Dinophyceae), a new species of Tovelliaceae with spiny cysts
复制标题
woloszynskioid 甲藻的研究 VI：Tovellia aveirensis sp. 的描述。
DOI：
10.1080/09670262.2014.910610
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
A. Calado
中科院分区：
文献类型：
--
作者：
Mariana S. Pandeirada;S. C. Craveiro;N. Daugbjerg;Ø. Moestrup;A. Calado
利用光镜（LM）、扫描电镜（SEM）、鞭毛细胞透射电镜（TEM）和大亚基核糖体rRNA基因部分序列的系统发育分析，描述了一种新的Tovellia， T. aveirensis。在阿威罗大学校园的一个水箱中建立的培养物中，研究了营养细胞和涉及有性生殖和静止囊肿产生的生命周期的几个阶段。营养细胞呈圆形，背腹侧受压较小；平合子较长，有一个比例较大的锥。透射电镜显示叶绿体裂片从中心的，分支的类pyrenoid放射，尽管这在LM上很难确定。鞭毛细胞的amphiesma主要由5或6面小泡组成，小泡具有薄板，在表锥上沿纬度排列5 - 7，在下锥上沿纬度排列3-5。扣带有2排板，后一排延伸至下锥体，并由一系列沿扣带下缘的小突起突起交叉。胞壁：一列狭窄的圆片，从腹侧的带附近延伸到胞顶背侧的中间。8或9个狭窄的两角形板排列在板的顶端线（ALP）的两侧。静止的包囊与之前描述的包囊不同之处在于，包囊有许多长而细的刺，其分叉的尖端分布在大部分表面。大多数成熟囊肿呈赤道缩窄。在培养的任何阶段均未见囊肿和运动细胞积聚红色细胞质体。系统发育分析表明，该新种属于托维利亚属（Tovellia）；在中等支持下，它与以其变红的细胞而著称的种血蛛形成了一个分支。
A new species of Tovellia, T. aveirensis, is described on the basis of light (LM) and scanning electron microscopy (SEM) of motile cells and resting cysts, complemented with transmission electron microscopy (TEM) of flagellate cells and phylogenetic analysis of partial sequences of the large subunit ribosomal rRNA gene. Both vegetative cells and several stages of a life cycle involving sexual reproduction and the production of resting cysts were examined in cultures established from a tank in the University of Aveiro campus. Vegetative cells were round and little compressed dorsoventrally; planozygotes were longer and had a proportionally larger epicone. Chloroplast lobes were shown by TEM to radiate from a central, branched pyrenoid, although this was difficult to ascertain in LM. The amphiesma of flagellate cells had mainly 5 or 6-sided vesicles with thin plates, arranged in 5–7 latitudinal series on the epicone, 3–5 on the hypocone. The cingulum had 2 rows of plates, the posterior row extending into the hypocone and crossed by a series of small projecting knobs along the lower edge of the cingulum. A line of narrow amphiesmal plates extended over the cell apex, from near the cingulum on the ventral side to the middle of the dorsal side of the epicone. Eight or 9 narrow amphiesmal plates lined each side of this apical line of plates (ALP). Resting cysts differed from any described before in having numerous long, tapering spines with branched tips distributed over most of the surface. Most mature cysts showed an equatorial constriction. Neither cysts nor motile cells were seen to accumulate red cytoplasmic bodies in any stage of the cultures. The phylogenetic analysis placed, with high statistical support, the new species within the genus Tovellia; it formed a clade, with moderate support, with T. sanguinea, a species notable for its reddening cells.