[Research on the blood components detecting by multi-optical path length spectroscopy technique].
[Research on the blood components detecting by multi-optical path length spectroscopy technique].
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发表时间：
2010-09
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通讯作者：
Gang Li;Zhe Zhao;Rui-An Liu;Hui-quan Wang;Hong-jie Wu;Ling Lin
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作者：
Gang Li;Zhe Zhao;Rui-An Liu;Hui-quan Wang;Hong-jie Wu;Ling Lin
为探讨利用血清多光程光谱信息测量人体血液成分浓度的可行性，设计了自动微位移测量装置，可获取200份血清样品的0~4.0 mm（间隔为0.2 mm）近红外多光程光谱，并以血清多光程光谱参与构建人体血液四种成分：葡萄糖（GLU）、总胆固醇的定量分析模型。 (TC)、总蛋白(TP)和白蛋白(ALB)，通过血液显着的非线性光谱特征。采用偏最小二乘法(PLS)，针对160个实验样品的生化分析结果，建立了多光程近红外吸收光谱的校准模型。根据该模型预测了另外40份样本的血液成分。 4种血液成分的预测效果良好，预测值与生化分析值的相关系数（r）分别为0.9320、0.9712、0.9462和0.9483。所有结果证明了多光程光谱技术用于血液成分分析的可行性。该技术为方便、快速地检测血液及其他液体成分奠定了基础。
To discuss the feasibility of using the serum's multi-optical path length spectroscopy information for measuring the concentration of the human blood components, the automatic micro-displacement measuring device was designed, which can obtain the near-infrared multi-optical path length from 0 to 4.0 mm (interval is 0.2 mm) spectra of 200 serum samples with multioptical path length spectrum of serum participated in building the quantitative analysis model of four components of the human blood: glucose (GLU), total cholesterol (TC), total protein (TP) and albumin (ALB), by mean of the significant non-linear spectral characteristic of blood. Partial least square (PLS) was used to set up the calibration models of the multi-optical path length near-infrared absorption spectrum of 160 experimental samples against the biochemical analysis results of them. The blood components of another 40 samples were predicted according to the model. The prediction effect of four blood components was favorable, and the correlation coefficient (r) of predictive value and biochemical analysis value were 0.9320, 0.9712, 0.9462 and 0.9483, respectively. All of the results proved the feasibility of the multi-optical path length spectroscopy technique for blood components analysis. And this technique established the foundation of detecting the components of blood and other liquid conveniently and rapidly.