SURFACTANT PROTEIN-B IN HUMAN FETAL LUNG - DEVELOPMENTAL AND GLUCOCORTICOID REGULATION
SURFACTANT PROTEIN-B IN HUMAN FETAL LUNG - DEVELOPMENTAL AND GLUCOCORTICOID REGULATION
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DOI：
10.1203/00006450-199511000-00007
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发表时间：
1995-11-01
影响因子：
3.6
通讯作者：
BALLARD, PL
中科院分区：
文献类型：
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作者：
BEERS, MF;SHUMAN, H;BALLARD, PL
Pulmonary surfactant protein B (SP-B) enhances phospholipid film formation in vitro and is essential for normal surfactant function in vivo. We examined human fetal lung before and during explant culture for content and cellular localization of SP-B mRNA and protein. SP-B mRNA was low in preculture specimens (18-20 wk) but hybridization signal increased over epithelial cells during culture and was enhanced by dexamethasone treatment (10 nM). SP-B immunofluorescence was very low in preculture specimens, increased during culture, and was uniformly intense in epithelial cells of dexamethasone-treated tissue. With a newly developed immunoassay, SP-B protein was undetectable in preculture lung (
