Comparison of Genomes of Brucella melitensis M28 and the B. melitensis M5-90 Derivative Vaccine Strain Highlights the Translation Elongation Factor Tu Gene tuf2 as an Attenuation-Related Gene
Comparison of Genomes of Brucella melitensis M28 and the B. melitensis M5-90 Derivative Vaccine Strain Highlights the Translation Elongation Factor Tu Gene tuf2 as an Attenuation-Related Gene
复制标题
羊布鲁氏菌 M28 和羊布鲁氏菌 M5-90 衍生疫苗株的基因组比较凸显了翻译延伸因子 Tu 基因 tuf2 作为减毒相关基因
DOI：
10.1128/iai.00224-13
复制
发表时间：
2013-08-01
影响因子：
3.1
通讯作者：
Bu, Zhigao
中科院分区：
文献类型：
--
作者：
Wang, Fangkun;Qiao, Zujian;Bu, Zhigao
羊种布鲁氏菌引起布鲁氏菌病，一种影响羊、牛，有时也影响人类的疾病。减毒B。羊种布氏杆菌M5-90株是由M28强毒株衍生而成的一种活疫苗，在我国反刍动物中广泛使用。菌株之间的遗传差异可能有助于阐明减毒机制。我们最近报道了M28和M5-90的全基因组序列。基因组结构在这些分离株之间是高度保守的，并且与强毒株16 M和ATCC 23457也是如此。分析显示23个开放阅读框（ORF），M5-90和强毒株之间存在一致差异。值得注意的是，与M28或其他强毒株的tuf 2相比，来自M5-90的编码翻译延伸因子EF-Tu的tuf 2基因含有50个单核苷酸多态性（SNP）和9个缺口（indel）。tuf 1没有变化。为了评估EF-Tu在发病机制中的潜在作用，构建了M28的tuf 1和tuf 2突变体以及携带野生型tuf 2的M5-90菌株，并在体内评估了它们的毒力/减毒作用。我们报告tuf 2基因在M5-90毒力减弱中起重要作用。
Brucella melitensis causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. Attenuated B. melitensis strain M5-90, derived from virulent strain M28, is widely used as a live vaccine in ruminants in China. Genetic differences between the strains may cast light on the mechanism of attenuation. We recently reported the complete genomic sequences of M28 and M5-90. Genome organization is highly conserved between these isolates, and also with virulent strains 16 M and ATCC 23457. Analysis revealed 23 open reading frames (ORFs) with consistent differences between M5-90 and the virulent strains. Notably, the tuf2 gene encoding translation elongation factor EF-Tu from M5-90 contained 50 single nucleotide polymorphisms (SNPs) and 9 gaps (indels) compared to tuf2 of M28 or of the other virulent strains. There were no changes in tuf1. To evaluate the potential role of EF-Tu in pathogenesis, tuf1 and tuf2 mutants of M28 and an M5-90 strain harboring wild-type tuf2 were constructed, and their virulence/attenuation was evaluated in vivo. We report that the tuf2 gene plays an important role in the attenuation of M5-90 virulence.