A Simple and Effective Method for Observing Starch in Whole Plant Cells and in Raw and Processed Food Ingredients
A Simple and Effective Method for Observing Starch in Whole Plant Cells and in Raw and Processed Food Ingredients
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DOI：
10.1002/star.202000056
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发表时间：
2020-11-02
期刊：
影响因子：
2.3
通讯作者：
Edwards，Cathrina H.
中科院分区：
文献类型：
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作者：
Parker，Mary L.;Ryden，Peter;Edwards，Cathrina H.
描述了一种使用环己烷二胺四乙酸（CDTA）从植物组织中产生大量分离的全细胞的方法。 CDTA 螯合二价阳离子，交联中间层的果胶多糖，从而无需严酷的物理处理即可温和地分离细胞。这些单个细胞非常适合通过显微镜原位观察淀粉颗粒，无需固定、包埋、切片或预先提取淀粉。淀粉可以很容易地观察到，无论是未染色的，还是通过偏振光学器件，或者用碘化物 (I2/KI) 染色后的淀粉。 I2/KI 染色结合偏振光学提供了偏振颜色信息，表明颗粒内的成分差异。显示了发育中、成熟和煮熟的皱纹豌豆细胞以及香蕉和马铃薯组织中淀粉补充的示例。 CDTA 分离方法非常适合研究淀粉突变体和其他细胞成分，因为它可以保留细胞质组织并防止储存过程中的微生物降解。
A method is described which uses cyclohexanediaminetetraacetic acid (CDTA) to produce numerous separated whole cells from plant tissue. CDTA chelates divalent cations that cross‐link the pectic polysaccharides of the middle lamella, allowing gentle separation of the cells without harsh physical treatments. These individual cells are ideal for observing starch granules in situ by microscopy without the requirement for fixation, embedding, sectioning, or prior starch extraction. Starch can easily be observed either unstained, or by polarizing optics, or after staining with iodide (I2/KI). Staining with I2/KI in combination with polarizing optics gives information on polarizing colors that indicate compositional differences within granules. Examples of the starch complement in developing, mature, and cookedrrwrinkled pea cells, and in banana and potato tissue are shown. The CDTA‐separation method is ideal for the survey of starch mutants and other cell components as it preserves cytoplasmic organization and prevents microbial degradation during storage.