Profiling of the cell surface proteome
Profiling of the cell surface proteome
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DOI：
10.1002/pmic.200300563
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发表时间：
2003-10-01
期刊：
影响因子：
3.4
通讯作者：
Hanash, S
中科院分区：
文献类型：
--
作者：
Jang, JH;Hanash, S
蛋白质组的深入挖掘需要对单个亚细胞区室中的蛋白质进行全面分析，以揭示蛋白质表达的有趣模式，包括评估蛋白质的位置、运输和位置特异性的翻译后修饰。从诊断和治疗的观点来看，非常感兴趣的隔室之一是质膜，其含有内在膜蛋白和在细胞表面上表达的其它蛋白。目前可用于细胞表面蛋白质组的全面分析的技术依赖于完整细胞的蛋白质标记。研究表明，细胞表面上的特定蛋白质的表达出现了意想不到的模式，以前认为主要发生在其他隔室，特别是内质网的蛋白质的常见情况。细胞表面和质膜蛋白质组的分析可能会提供新的见解，并揭示疾病相关的变化。
The in depth-mining of the proteome necessitates the comprehensive analysis of proteins in individual subcellular compartments to uncover interesting patterns of protein expression that include assessment of protein location, trafficking and of post-translational modifications that are location specific. One of the compartments of substantial interest from a diagnostic and therapeutic point of view is the plasma membrane which contains intrinsic membrane proteins and other proteins expressed on the cell surface. Technologies are currently available for the comprehensive profiling of the cell surface proteome that rely on protein tagging of intact cells. Studies are emerging that point to unexpected patterns of expression of specific proteins on the cell surface, with a common occurrence of proteins previously considered to occur predominantly in other compartments, notably the endoplasmic reticulum. The profiling of the cell surface and plasma membrane proteomes will likely provide novel insights and uncover disease related alterations.