An animal model study for tissue-engineered trachea fabricated from a biodegradable scaffold using chondrocytes to augment repair of tracheal stenosis
An animal model study for tissue-engineered trachea fabricated from a biodegradable scaffold using chondrocytes to augment repair of tracheal stenosis
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DOI：
10.1016/j.jpedsurg.2008.08.038
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发表时间：
2008-12-01
影响因子：
2.4
通讯作者：
Iwanaka, Tadashi
中科院分区：
文献类型：
--
作者：
Komura, Makoto;Komura, Hiroko;Iwanaka, Tadashi
简介：我们设计了一种使用软骨细胞由可生物降解支架制成的工程移植物，并应用该结构来增强气管狭窄的修复。本研究探讨了使用这种组织工程气道与自体软骨细胞在兔model.Material和方法的可行性：软骨细胞从新西兰白色兔耳软骨分离和扩增，然后种植在复合3层支架组成的胶原片，聚乙醇酸网，和共聚物（L-丙交酯/ε-己内酯）粗网。将工程移植物植入在颈部气管中腹侧部分创建的0.5 x 0.8 cm缺损中。明胶海绵，缓慢释放碱性成纤维细胞生长因子（b-FGF），然后放置油的结构，这是收回1或3个月后implantation.Results：种植软骨细胞的生物可降解支架可以维持气道结构，至3个月后植入。气管上皮再生发生在这种复合支架的内腔中。结论：该复合支架材料具有良好的生物降解性，可用于构建工程化气管。明胶海绵缓慢释放b-FGF可能会增强软骨形成。(C)2008年爱思唯尔公司All rights reserved.
Introduction: We have designed an engineered graft fabricated from a biodegradable scaffold using chondrocytes and applied this construct to augment repair of tracheal stenosis. This Study investigated the feasibility of using Such tissue-engineered airways with autologous chondrocytes in a rabbit model.Material and Methods: Chondrocytes were isolated and expanded from the auricular cartilage of New Zealand white rabbits, then seeded onto composite 3-layer scaffolds consisting of a collagen sheet, a polyglycolic acid mesh, and a copolymer (L-lactide/epsilon-caprolactone) coarse mesh. The engineered grafts were implanted into a 0.5 x 0.8-cm defect created in the midventral portion of the cervical trachea. Gelatin sponges that slowly released basic fibroblast growth factor (b-FGF) were then placed oil the constructs, which were retrieved I or 3 months after implantation.Results: The biodegradable scaffold seeded with chondrocytes could maintain airway structure up to 3 months after implantation. Tracheal epithelial regeneration occurred in the internal lumen of this composite scaffold. Three months after implantation, staining of the sections showed cartilage accumulation in the engineered tracheal wall.Conclusion: This composite biodegradable scaffold may be useftil for developing engineered trachea. A gelatin sponge slowly releasing b-FGF might enhance chondrogenesis. (C) 2008 Elsevier Inc. All rights reserved.