Phage Display Selection of Cyclic Peptides That Inhibit Andes Virus Infection
Phage Display Selection of Cyclic Peptides That Inhibit Andes Virus Infection
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DOI：
10.1128/jvi.00606-09
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发表时间：
2009-09-01
影响因子：
5.4
通讯作者：
Larson, Richard S.
中科院分区：
文献类型：
--
作者：
Hall, Pamela R.;Hjelle, Brian;Larson, Richard S.
对于由安第斯山脉病毒（ANDV）引起的汉坦病毒心肺综合征，尚无特异性治疗方法。利用抗ANDV表面糖蛋白Gn和Gc的抗体，从纯化的ANDV颗粒中竞争性地扩增携带环状九肽的噬菌体展示文库，鉴定了能够在体外阻断ANDV感染的肽。在体外检测噬菌体对ANDV感染的抑制作用，并基于最有效的噬菌体序列合成九肽。三种肽显示出病毒抑制水平，其通过与抗Gn-和抗Gc-靶向肽的组合治疗而显著增加。这些肽将是进一步开发肽和非肽治疗剂的有价值的工具。
Specific therapy is not available for hantavirus cardiopulmonary syndrome caused by Andes virus (ANDV). Peptides capable of blocking ANDV infection in vitro were identified using antibodies against ANDV surface glycoproteins Gn and Gc to competitively elute a cyclic nonapeptide-bearing phage display library from purified ANDV particles. Phage was examined for ANDV infection inhibition in vitro, and nonapeptides were synthesized based on the most-potent phage sequences. Three peptides showed levels of viral inhibition which were significantly increased by combination treatment with anti-Gn- and anti-Gc-targeting peptides. These peptides will be valuable tools for further development of both peptide and nonpeptide therapeutic agents.