Estimates of gene flow in Drosophila pseudoobscura determined from nucleotide sequence analysis of the alcohol dehydrogenase region.
Estimates of gene flow in Drosophila pseudoobscura determined from nucleotide sequence analysis of the alcohol dehydrogenase region.
复制标题
通过醇脱氢酶区域的核苷酸序列分析确定果蝇中的基因流估计。
DOI：
10.1093/genetics/132.2.471
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发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Miller,EL
中科院分区：
文献类型：
--
作者：
Schaeffer,SW;Miller,EL
通过对果蝇乙醇脱氢酶(ADH)区3.4kb片段的核苷酸序列分析，推测了果蝇种群的遗传结构。从北美和南美的13个种群收集的99个等染色体菌株被用来确定是否有任何种群偏离了中性模型，并估计了种群之间的基因流动水平。这项研究还包括了两个近缘物种D.persimilis和D.Miranda的核苷酸序列。我们估计了9个样本大小大于3的9个群体中17个ADH亚区同义和非编码区的中性突变参数4Nu。对九个种群的核苷酸多样性数据进行了两次统计检验，以确定是否偏离了平衡中性模型。Tajima和Hudson，Kreitman，Aguade测试表明，每个群体都未能拒绝中性模型。种群间的遗传分化测试没有显示北美种群间的任何种群亚结构。核苷酸多样性数据与基因流动的直接和间接测量相一致，这些测量表明假黑斑潜蝇在种群之间广泛传播。
The genetic structure of Drosophila pseudoobscura populations was inferred from a nucleotide sequence analysis of a 3.4-kb segment of the alcohol dehydrogenase (Adh) region. A total of 99 isochromosomal strains collected from 13 populations in North and South America were used to determine if any population departed from a neutral model and to estimate levels of gene flow between populations. This study also included the nucleotide sequences from two sibling species, D. persimilis and D. miranda. We estimated the neutral mutation parameter, 4N mu, in synonymous and noncoding sites for 17 subregions of Adh in each of nine populations with sample sizes greater than three. The nucleotide diversity data in the nine populations was tested for departures from an equilibrium neutral model with two statistical tests. The Tajima and the Hudson, Kreitman, Aguade tests showed that each population fails to reject a neutral model. Tests for genetic differentiation between populations fail to show any population substructure among the North American populations of D. pseudoobscura. The nucleotide diversity data is consistent with direct and indirect measures of gene flow that show extensive dispersal between populations of D. pseudoobscura.