Structure of the 80S ribosome from Saccharomyces cerevisiae -: tRNA-ribosome and subunit-subunit interactions
Structure of the 80S ribosome from Saccharomyces cerevisiae -: tRNA-ribosome and subunit-subunit interactions
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DOI：
10.1016/s0092-8674(01)00539-6
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发表时间：
2001-11-02
期刊：
影响因子：
64.5
通讯作者：
Frank, J
中科院分区：
文献类型：
--
作者：
Spahn, CMT;Beckmann, R;Frank, J
分析了翻译酵母80S核糖体的冷冻-EM重建。计算分离的rRNA和蛋白质密度用于对接适当修饰的rRNA模型和酵母核糖体蛋白的同源性模型。核糖体的核心显示出显著的保守性。然而，由于扩展片段和额外的核糖体蛋白，在功能上重要的区域和外周的显着变化的一些显着差异是显而易见的。在细菌核糖体中，亚基之间的桥主要由RNA接触形成。四座新桥位于外围。P位点tRNA的位置与其原核对应物完全一致，主要是rRNA对其分子环境有贡献。这种分析在分子水平上提供了真核生物核糖体的详尽清单。
A cryo-EM reconstruction of the translating yeast 80S ribosome was analyzed. Computationally separated rRNA and protein densities were used for docking of appropriately modified rRNA models and homology models of yeast ribosomal proteins. The core of the ribosome shows a remarkable degree of conservation. However, some significant differences in functionally important regions and dramatic changes in the periphery due to expansion segments and additional ribosomal proteins are evident. As in the bacterial ribosome, bridges between the subunits are mainly formed by RNA contacts. Four new bridges are present at the periphery. The position of the P site tRNA coincides precisely with its prokaryotic counterpart, with mainly rRNA contributing to its molecular environment. This analysis presents an exhaustive inventory of an eukaryotic ribosome at the molecular level.